小鼠IL-1ra ELISA试剂盒(ab113348)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IL-1ra ELISA试剂盒
参阅全部 IL-1RA 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
2.74 pg/ml - 2000 pg/ml -
回收率
90 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 102 94% - 103% Serum 75.89 67% - 84% Plasma 93.69 71% - 111% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse
预测可用于: Rat, Human
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产品概述
Abcam’s IL-1ra Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-1ra in serum, plasma (collect plasma using EDTA and heparin as an anticoagulant. Citrate is not recommended for this assay) and cell culture supernates.
This assay employs an antibody specific for mouse IL-1ra coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1ra present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1ra antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1ra bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-Mouse IL-1ra 2 vials IL-1ra Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-1ra Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. -
组织特异性
The intracellular form of IL1RN is predominantly expressed in epithelial cells. -
疾病相关
Microvascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency -
细胞定位
Cytoplasm and Secreted. - Information by UniProt
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别名
- DIRA
- F630041P17Rik
- ICIL 1RA
see all -
数据库链接
- Entrez Gene: 3557 Human
- Entrez Gene: 16181 Mouse
- Entrez Gene: 60582 Rat
- Omim: 147679 Human
- SwissProt: P18510 Human
- SwissProt: P25085 Mouse
- SwissProt: P25086 Rat
- Unigene: 81134 Human
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113348于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Sandwich ELISA - IL-1ra Mouse ELISA Kit (ab113348)Standard curve of msIL-1Ra in different diluents with background signal subtracted (duplicates; +/- SD).
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Sandwich ELISA - IL-1ra Mouse ELISA Kit (ab113348)IL-1Ra measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD). -
Sandwich ELISA - IL-1ra Mouse ELISA Kit (ab113348)msIL-1Ra measured in biological fluids, background signal subtracted (duplicates +/- SD). -
Sandwich ELISA - IL-1ra Mouse ELISA Kit (ab113348)IL-1Ra detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD). -
Typical Standard CurveRepresentative Standard Curve using ab113348
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Typical Standard CurveRepresentative Standard Curve using ab113348
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab113348 被引用在 2 文献中.
- Xia Q et al. SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury. iScience 26:106953 (2023). PubMed: 37332598
- Sakkou M et al. Mesenchymal TNFR2 promotes the development of polyarthritis and comorbid heart valve stenosis. JCI Insight 3:N/A (2018). PubMed: 29618659