小鼠IL-13 ELISA试剂盒(ab219634)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.2 pg/ml
- Range: 1.95 pg/ml - 125 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IL-13 ELISA试剂盒
参阅全部 IL-13 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% supernatant 5 2.2% 批次间 样品 n Mean SD CV% supernatant 3 1.4% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1.2 pg/ml -
范围
1.95 pg/ml - 125 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 108 106% - 111% Serum 97 95% - 98% Hep Plasma 104 101% - 108% EDTA Plasma 98 97% - 99% Cit plasma 97 95% - 100% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse -
产品概述
Mouse IL-13 ELISA Kit (ab219634) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-13 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-13 with 1.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse IL-13 Capture Antibody 1 x 600µl 10X Mouse IL-13 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR2 1 x 6ml Mouse IL-13 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
疾病相关
Allergic rhinitis -
序列相似性
Belongs to the IL-4/IL-13 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Allergic rhinitis
- ALRH
- BHR 1
see all -
数据库链接
- Entrez Gene: 16163 Mouse
- SwissProt: P20109 Mouse
- Unigene: 1284 Mouse
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab219634于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Other - Mouse IL-13 ELISA Kit (ab219634)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse IL-13 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike IL-13 in mouse serum and plasma samples.The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native IL-13 in mouse serum and plasma samples.The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13 concentration was determined to be 31.5 pg/mL in serum, 21.1 pg/mL in plasma (citrate) and 20.4 pg/mL in plasma (EDTA) and 29.1 pg/mL in plasma (heparin).
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Interpolated concentrations of native IL-13 in mouse EL4/IL-2 cell culture supernatant unstimulated and PHA/PMA stimulated samples.The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). T The mean IL-13 concentration was determined to be 3144 pg/mL in PHA/PMA stimulated EL4/IL-2 cell culture supernatant and undetermined in media and unstimulated cell culture supernatant.
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Sandwich ELISA - Mouse IL-13 ELISA Kit (ab219634)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab219634 被引用在 20 文献中.
- Mo S et al. Chryseriol attenuates the progression of OVA-induced asthma in mice through NF-κB/HIF-1α and MAPK/STAT1 pathways. Allergol Immunopathol (Madr) 51:146-153 (2023). PubMed: 36617834
- Xia Q et al. SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury. iScience 26:106953 (2023). PubMed: 37332598
- He R et al. Mechanism of miR-181a-5p in Regulatory T/T-Helper 17 Immune Imbalance and Asthma Development in Mice with Allergic Rhinitis. Int Arch Allergy Immunol 183:375-388 (2022). PubMed: 34942624
- Wang X et al. Pulsatilla decoction alleviates colitis by enhancing autophagy and regulating PI3K‑Akt‑mTORC1 signaling pathway. Mol Med Rep 25:N/A (2022). PubMed: 35103289
- Xiang J et al. Lidocaine relieves murine allergic rhinitis by regulating the NF-κB and p38 MAPK pathways. Exp Ther Med 23:193 (2022). PubMed: 35126696