小鼠IgG1 ELISA试剂盒(ab133045)
Key features and details
- Sensitivity: 0.116 ng/ml
- Range: 7.81 ng/ml - 250 ng/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IgG1 ELISA试剂盒
参阅全部 IgG1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Low 19 49.9ng/ml 1.2% Medium 19 99.6ng/ml 2.7% High 19 160.4ng/ml 3.6% 批次间 样品 n Mean SD CV% Low 16 48.5ng/ml 3.1% Medium 16 98.3ng/ml 4.4% High 16 148.7ng/ml 8.1% -
样品类型
Cell culture supernatant, Serum -
检测类型
Sandwich (quantitative) -
灵敏度
0.116 ng/ml -
范围
7.81 ng/ml - 250 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 102.8 % - % Tissue Culture Media 105.3 % - % -
检测时间
0h 90m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s Mouse IgG1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse IgG1 in Culture supernatants and Serum.
IgG1 specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated IgG1 detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the IgG1 amount of sample captured in plate.
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说明
IgG is divided into four subclasses; IgG1, IgG2, IgG3, and IgG4. IgG1 is the most abundant immunoglobulin found in the blood. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the “hinge region” which is unique to each IgG. This region allows for molecular flexibility and sets IgG1 apart from its IgG counterparts. IgG1 properties and functions include neutralization, opsonization, activation of the complement system, diffusion into extravascular sites and crossing the placenta.
Cross Reactivity
Compound Cross Reactivity Rat IgG1 0.9% Mouse IgG2b 0.21 Human IgG1 <0.01% Mouse IgG2a <0.01% Mouse IgG3 <0.01% Mouse IgM <0.01% Recommended dilution of serum is 1:20,000
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Please refer to protocols. -
组件 标识符 1 x 96 tests 20X Wash Buffer Concentrate 1 x 100ml Assay Buffer 13 Concentrate 1 x 50ml Goat anti-mouse IgG Microplate (12 x 8 wells) 1 unit Mouse IgG1 Conjugate Concentrate 1 x 0.07ml Mouse IgG1 Standard 1 x 0.25ml Mouse IgG1 Conjugate Diluent Blue 1 x 6ml Plate Sealer 2 units Stop Solution 2 1 x 11ml TMB Substrate 1 x 12ml -
研究领域
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相关性
There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). -
别名
- Ig gamma 1 chain C region
- IGHG1
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133045于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab133045 被引用在 8 文献中.
- Ortega-Molina A et al. Inhibition of Rag GTPase signaling in mice suppresses B cell responses and lymphomagenesis with minimal detrimental trade-offs. Cell Rep 36:109372 (2021). PubMed: 34260908
- Moore EM et al. Biomaterials direct functional B cell response in a material-specific manner. Sci Adv 7:eabj5830 (2021). PubMed: 34851674
- Hao L et al. Combinational PRR Agonists in Liposomal Adjuvant Enhances Immunogenicity and Protective Efficacy in a Tuberculosis Subunit Vaccine. Front Immunol 11:575504 (2020). PubMed: 33117374
- Ullah N et al. Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis Infection. J Immunol Res 2020:2083793 (2020). PubMed: 32953889
- Ortega-Molina A et al. Oncogenic Rag GTPase signaling enhances B cell activation and drives follicular lymphoma sensitive to pharmacological inhibition of mTOR. Nat Metab 1:775-789 (2019). PubMed: 31579886
- Singh NK et al. Anti-allergy and anti-tussive activity of Clitoria ternatea L. in experimental animals. J Ethnopharmacol 224:15-26 (2018). PubMed: 29787795
- Ma J et al. A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation. EBioMedicine 22:143-154 (2017). PubMed: 28711483
- Hu B et al. Development of a reverse genetics system for respiratory syncytial virus long strain and an immunogenicity study of the recombinant virus. Virol J 11:142 (2014). PubMed: 25107552