小鼠IgG ELISA试剂盒- 30 minutes (ab273150)
Key features and details
- Sensitivity: 6 ng/ml
- Range: 20 ng/ml - 1900 ng/ml
- Sample type: Ascites Fluid, Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
概述
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产品名称
小鼠IgG ELISA试剂盒- 30 minutes
参阅全部 IgG 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 1/100 9 10.63µg/ml 5.33 % B 1/100 9 11.56µg/ml 5.33 % C 1/100 9 22.61pg/ml 8.48 % D 1/100 9 28.88µg/ml 10.03 % E 1/100 9 66.82µg/ml 8.39 % F 1/100 9 75.92µg/ml 9.9 % G 1/100 9 102.47µg/ml 10.38 % 批次间 样品 n Mean SD CV% H 1/250 30 3.45 % H 1/500 30 2.99 % H 1/1000 30 4.97 % -
样品类型
Cell culture supernatant, Ascites Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
6 ng/ml -
范围
20 ng/ml - 1900 ng/ml -
实验步骤
One step assay -
产品概述
The Mouse IgG quantification Kit (ab273150) provides a rapid and easy method (one step ELISA) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid.
The kit includes ready-to-use reagents necessary to analyze up to 90 samples in 30 min. Buffer solutions are color coded in order to simplify pipetting steps.
A polyclonal antibody specific to mouse IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and mouse IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mouse IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 10 x 96 tests 1 x 96 tests Detection antibody 1 x 120ml 1 x 12ml Mouse IgG standard 0.0 µg/ml 1 x 1ml 1 x 0.3ml Mouse IgG standard 0.02 µg/ml 1 x 1ml 1 x 0.3ml Mouse IgG standard 0.1 µg/ml 1 x 1ml 1 x 0.3ml Mouse IgG standard 0.2 µg/ml 1 x 1ml 1 x 0.3ml Mouse IgG standard 0.75 µg/ml 1 x 1ml 1 x 0.3ml Mouse IgG standard 1.9 µg/ml 1 x 1ml 1 x 0.3ml Pre-coated 8 microwells strips 10 x 96 tests 1 x 96 tests Sample Diluent 1 x 500ml 1 x 30ml Stop Solution 1 x 120ml 1 x 12ml TMB Substrate 1 x 120ml 1 x 12ml -
研究领域
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细胞定位
Secreted -
别名
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273150于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Mouse Immunoglobulin Quantification Standard curve
Example of Mouse IgG quantitative kit Standard curve
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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