小鼠HGF ELISA试剂盒(ab223862)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.4 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
-
产品名称
小鼠HGF ELISA试剂盒
参阅全部 HGF 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 5 3.2% 批次间 样品 n Mean SD CV% Serum 3 4.9% -
样品类型
Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
8.4 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 111 109% - 117% Cell culture extracts 104 101% - 107% Tissue Extracts 119 113% - 123% Cell culture media 109 107% - 112% EDTA Plasma 104 100% - 108% Cit plasma 108 105% - 111% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse -
产品概述
Mouse HGF ELISA Kit (ab223862) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HGF protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse HGF with 8.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
说明
Hepatocyte Growth Factor (HGF) is mitogen of parenchymal hepatocytes. HGF also acts as a growth factor and is expressed by a wide variety of tissue and cell types. HGF binds to the HGF- Receptor to help regulate cell growth, cell motility and morphogenesis. Elevated circulating levels of HGF can be measured as prognostic marker of severity of hypertension and heart failure. HGF is secreted as a full length inactive polypeptide, and then is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta chain which dimerize producing the active heterodimeric molecule. Mouse HGF SimpleStep ELISA® (ab223862) is designed to recognize this dimerized protein.
The standard is affinity purified.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse HGF Capture Antibody 1 x 600µl 10X Mouse HGF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR2 1 x 6ml Mouse HGF Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
-
功能
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. -
疾病相关
Deafness, autosomal recessive, 39 -
序列相似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
-
别名
- DFNB39
- F TCF
- Fibroblast derived tumor cytotoxic factor
see all -
数据库链接
- Entrez Gene: 15234 Mouse
- SwissProt: Q08048 Mouse
- Unigene: 267078 Mouse
相关产品
-
Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223862于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
-
Other - Mouse HGF ELISA Kit (ab223862)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Mouse HGF standard curve comparison dataStandard curve comparison between mouse HGF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
-
Example of mouse HGF standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
-
Example of mouse HGF standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native HGF in mouse serum and plasma samplesThe concentrations of HGF were measured in duplicates, interpolated from the HGF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (EDTA) 50%, plasma (citrate) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 11,764 pg/mL in serum, 1099 pg/mL in plasma (EDTA), 691 pg/mL in plasma (citrate) and 896 pg/mL in plasma heparin).
-
Interpolated concentrations of native HGF in mouse cell and tissue extracts, extracts based on a 4,000 µg/mL extract loadThe concentrations of HGF were measured in duplicate and interpolated from the HGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 7,859 pg/mL in NIH/3T3 cell extract, 2,472 pg/mL in mouse spleen tissue extract and 1,291 pg/mL in mouse kidney tissue extract.
-
Interpolated concentrations of native HGF in mouse 3T3-L1 undifferentiated and 3T3-L1 adipocyte differentiated cell extract samples, sample based on a 1,000 µg/mL extract loadMouse 3T3-L1 cells were either undifferentiated (not allowed to reach confluency) or differentiated (cultured for 48 hours in adipocyte differentiation medium). The concentrations of HGF were measured in duplicate and interpolated from the HGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 2,636 pg/mL in undifferentiated 3T3-L1 cell extract and 88 pg/mL in adipocyte differentiated 3T3-L1 cell extract.
-
Interpolated concentrations of native HGF in rat serum and plasma samplesThe concentrations of HGF were measured in duplicates, interpolated from the HGF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (EDTA) 100%, plasma (citrate) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 79,543 pg/mL in serum, 3,151 pg/mL in plasma (EDTA), 2,395 pg/mL in plasma (citrate) and 2,611 pg/mL in plasma heparin).
-
Recombinant rat and mouse HGF standard curvesRecombinant rat HGF was prepared at 4 ng/mL and diluted with a 2-fold titration alongside of mouse HGF standard curve, and assayed for cross reactivity. 100% cross-reactivity was observed.
-
Sandwich ELISA - Mouse HGF ELISA Kit (ab223862)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab223862 被引用在 2 文献中.
- Yang X et al. Endoplasmic reticulum stress modulates the fate of lung resident mesenchymal stem cell to myofibroblast via C/EBP homologous protein during pulmonary fibrosis. Stem Cell Res Ther 13:279 (2022). PubMed: 35765096
- Zhang W et al. Toll-like receptor 5-mediated signaling enhances liver regeneration in mice. Mil Med Res 8:16 (2021). PubMed: 33622404