小鼠Haptoglobin ELISA试剂盒
Mouse Haptoglobin ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
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Mouse Haptoglobin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Haptoglobin with a sensitivity of 3.91 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Haptoglobin, Hp
- ELISA
Supplier Data
ELISA - Mouse Haptoglobin ELISA Kit (AB272472)
- sELISA
Supplier Data
Sandwich ELISA - Mouse Haptoglobin ELISA Kit (AB272472)
Interpolated concentrations of native Haptoglobin in mouse serum and plasma samples.
The concentrations of Haptoglobin were measured in duplicates, interpolated from the Haptoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 250,000, plasma (citrate) 1 : 1,000,000, plasma (EDTA) 1 : 250,000, and plasma (heparin) 1 : 250,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Haptoglobin concentration was determined to be 271.1 μg/mL in serum, 836.8 μg/mL in plasma (citrate), 265.2 μg/mL in plasma (EDTA), and 282.0 μg/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Mouse Haptoglobin ELISA Kit (AB272472)
Example of mouse Haptoglobin standard curve in Sample Diluent NS.
The Haptoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Haptoglobin ELISA Kit (AB272472)
Interpolated concentrations of native Haptoglobin in mouse tissue culture supernatant and urine samples.
The concentrations of Haptoglobin were measured in duplicates, interpolated from the Haptoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows : liver supernatant 1 : 200 and urine 1 : 160. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Haptoglobin concentration was determined to be 211.7 ng/mL in liver supernatant and 109.3 ng/mL in urine.
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反应性数据
产品详情
Mouse Haptoglobin ELISA Kit ab272472 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Haptoglobin in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 3.91 pg/mL.
How the assay works
Mouse Haptoglobin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse Haptoglobin ELISA Kit ab272472 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Haptoglobin antibody [EPR22856-71] - BSA and Azide free (Capture) ab272756
- Anti-Haptoglobin antibody [EPR22856-277] - BSA and Azide free (Detector) ab272756
Haptoglobin, encoded by the HP gene and produced mostly by hepatic cells, is a major acute phase protein. Haptoglobin is known to bind to free hemoglobin released from erythrocytes with high affinity and inhibits its oxidative activity to allow hepatic recycling of the heme iron. Biosynthesis of haptoglobin can also occur in adipose and lung tissues to provide antioxidant and antimicrobial activity. Studies have shown haptoglobin to play a role in stimulation of angiogenesis and changes in the measured concentrations in serum may associate with certain inflammations, infections, and hemolytic conditions. Human, rat, cow, monkey share 81%, 90%, 76%, and 80% sequence homology with mouse haptoglobin, respectively.
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回收率
规格
性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.
Pathways
The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.
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