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AB171178

小鼠GDNF ELISA试剂盒

Mouse GDNF ELISA Kit

5

(1 Review)

|

(7 Publications)

Mouse GDNF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse GDNF in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

查看别名

Glial cell line-derived neurotrophic factor, mGDNF, Astrocyte-derived trophic factor, ATF, Gdnf

1 Images
Functional Studies - Mouse GDNF ELISA Kit (AB171178)
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Functional Studies - Mouse GDNF ELISA Kit (AB171178)

Representative standard curve using ab171178.

关键信息

检测方法

Colorimetric

样品类型

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

反应种属

Mouse

检测类型

Sandwich (quantitative)

灵敏度

< 10 pg/mL

范围

31.2 - 2000 pg/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Abcam's mouse GDNF in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse GDNF in cell culture supernatants, serum and plasma (heparin, EDTA).

A GDNF specific goat polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for GDNF is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse GDNF amount of sample captured in plate.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab171178-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB171178-CMP06" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB171178-CMP05" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-mouse GDNF antibody", "number":"AB171178-CMP02" }, { "size":"1 x 1 Unit", "name":"Anti-mouse GDNF antibody Microplate (12 x 8 wells)", "number":"AB171178-CMP03" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB171178-CMP07" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB171178-CMP10" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB171178-CMP04" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB171178-CMP09" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB171178-CMP08" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant mouse GDNF standard", "number":"AB171178-CMP01" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB171178-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab171178-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB171178-CMP06", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant mouse GDNF standard", "number":"AB171178-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB171178-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB171178-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB171178-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB171178-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB171178-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse GDNF antibody Microplate (12 x 8 wells)", "number":"AB171178-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-mouse GDNF antibody", "number":"AB171178-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB171178-CMP04", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor. Involved in the development of the neural crest.
See full target information Gdnf
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 19:e3001350 PubMed34748545

2021

Adult medial habenula neurons require GDNF receptor GFRα1 for synaptic stability and function.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Fernández-Suárez,Favio A Krapacher,Katarzyna Pietrajtis,Annika Andersson,Lilian Kisiswa,Alvaro Carrier-Ruiz,Marco A Diana,Carlos F Ibáñez

International journal of molecular sciences 22: PubMed34445251

2021

Enhanced Nerve Regeneration by Exosomes Secreted by Adipose-Derived Stem Cells with or without FK506 Stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Shyuan Rau,Pao-Jen Kuo,Shao-Chun Wu,Lien-Hung Huang,Tsu-Hsiang Lu,Yi-Chan Wu,Chia-Jung Wu,Chia-Wei Lin,Chia-Wen Tsai,Ching-Hua Hsieh

Molecular therapy : the journal of the American Society of Gene Therapy 28:642-652 PubMed31495777

2019

SINEUP Non-coding RNA Targeting GDNF Rescues Motor Deficits and Neurodegeneration in a Mouse Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano Espinoza,Margherita Scarpato,Devid Damiani,Francesca Managò,Maddalena Mereu,Andrea Contestabile,Omar Peruzzo,Piero Carninci,Claudio Santoro,Francesco Papaleo,Federico Mingozzi,Giuseppe Ronzitti,Silvia Zucchelli,Stefano Gustincich

Glia 66:445-458 PubMed29105838

2017

A Parkinson's disease gene, DJ-1, repairs brain injury through Sox9 stabilization and astrogliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Joo Choi,Jin-Hwa Eun,Byung Gon Kim,Ilo Jou,Sang Myun Park,Eun-Hye Joe

Scientific reports 6:36779 PubMed27827452

2016

GDNF-expressing STO feeder layer supports the long-term propagation of undifferentiated mouse spermatogonia with stem cell properties.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Wei,Yuanyuan Jia,Yuanyuan Xue,Lei Geng,Min Wang,Lufan Li,Mei Wang,Xuemei Zhang,Xin Wu

Molecules (Basel, Switzerland) 21: PubMed27490526

2016

Agavins Increase Neurotrophic Factors and Decrease Oxidative Stress in the Brains of High-Fat Diet-Induced Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Franco-Robles,Mercedes G López

PloS one 9:e100196 PubMed24949623

2014

Morphine enhances HIV-1SF162-mediated neuron death and delays recovery of injured neurites.

Applications

Unspecified application

Species

Unspecified reactive species

Ruturaj R Masvekar,Nazira El-Hage,Kurt F Hauser,Pamela E Knapp
View all publications
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