AB203369

小鼠Galectin 3 ELISA试剂盒

Mouse Galectin 3 ELISA Kit

Recombinant
SimpleStep
了解详情

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(5 Publications)

Mouse Galectin 3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Galectin 3 with a sensitivity of 2.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

查看别名

Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, IgE-binding protein, L-34 galactoside-binding lectin, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, Lgals3

4 Images

ELISA - Mouse Galectin 3 ELISA Kit (AB203369)

ELISA

Supplier Data

ELISA - Mouse Galectin 3 ELISA Kit (AB203369)

sELISA

Supplier Data

Sandwich ELISA - Mouse Galectin 3 ELISA Kit (AB203369)

Comparison of secreted Galectin-3 in unstimulated and PMA/PHA-stimulated J774A.1 Cells.

J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Galectin-3 was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated J774A.1 and cell culture media. Measured values were interpolated from the Galectin-3 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 14 ng/mL unstimulated, 38.9 ng/mL stimulated, and undetectable in media.

sELISA

Supplier Data

Sandwich ELISA - Mouse Galectin 3 ELISA Kit (AB203369)

Interpolated concentrations of Galectin-3 in mouse serum, plasma (citrate), and plasma (EDTA).

The concentrations of Galectin-3 were measured in duplicate and interpolated from the Galectin-3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 11.3 ng/mL in mouse serum, 13.2 ng/mL in mouse plasma (Citrate), and 12.9 ng/mL in mouse plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse Galectin 3 ELISA Kit (AB203369)

Example of Galectin-3 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Mouse

检测类型

Sandwich

灵敏度

= 2.4 pg/mL

范围

7.81 - 500 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Mouse Galectin 3 ELISA Kit ab203369 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Galectin 3 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 2.4 pg/mL.

How the assay works

Mouse Galectin 3 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Galectin 3 ELISA Kit ab203369 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Galectin 3 antibody [EPR17811-106] - BSA and Azide free (Capture) ab242746
- Anti-Galectin 3 antibody [EPR17811-102] - BSA and Azide free (Detector) ab242746

Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is in the Galectin family of carbohydrate-binding proteins and is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

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回收率

[ { "sample": "Serum", "range": "105 - 108 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "107 - 115 %", "average": "= 111" }, { "sample": "Urine", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "104 - 111 %", "average": "= 108" }, { "sample": "Cell culture media", "range": "92 - 97 %", "average": "= 94" } ]

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.

Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like ?-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-?. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.

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产品实验方案

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靶点信息

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration (PubMed : 15181153). Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus : acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes (By similarity). When secreted, interacts with NK cell-activating receptor NCR3/NKp30 acting as an inhibitory ligand which antagonizes NK cell attack (By similarity).

See full target information Lgals3

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiovascular development and disease 9: PubMed36547420

2022

Combination of , and Improves Cardiac Functions and Reduces Pro-Inflammatory Biomarkers in Preclinical Models of Short-Term Doxorubicin-Mediated Cardiotoxicity: New Frontiers in Cardioncology?

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Quagliariello,Manuela Giovanna Basilicata,Giacomo Pepe,Raffaele De Anseris,Annabella Di Mauro,Giosuè Scognamiglio,Giuseppe Palma,Vincenzo Vestuto,Simona Buccolo,Antonio Luciano,Massimiliano Barbieri,Francesca Bruzzese,Carlo Maurea,Rossella Pumpo,Carmine Ostacolo,Pietro Campiglia,Massimiliano Berretta,Nicola Maurea

Biomolecules 12: PubMed35625551

2022

-GlcNAcylation and Regulation of Galectin-3 in Extraembryonic Endoderm Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed I Gatie,Danielle M Spice,Amritpal Garha,Adam McTague,Mariam Ahmer,Alexander V Timoshenko,Gregory M Kelly

Molecular therapy. Methods & clinical development 18:199-214 PubMed32671132

2020

Enzyme Replacement Therapy Can Reverse Pathogenic Cascade in Pompe Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Naresh Kumar Meena,Evelyn Ralston,Nina Raben,Rosa Puertollano

Arteriosclerosis, thrombosis, and vascular biology 40:1491-1509 PubMed32295421

2020

Galectin-3 Identifies a Subset of Macrophages With a Potential Beneficial Role in Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Di Gregoli,Michelle Somerville,Rosaria Bianco,Anita C Thomas,Aleksandra Frankow,Andrew C Newby,Sarah J George,Christopher L Jackson,Jason L Johnson

Frontiers in endocrinology 11:30 PubMed32117058

2020

Overexpression of Galectin 3 in Pancreatic β Cells Amplifies β-Cell Apoptosis and Islet Inflammation in Type-2 Diabetes in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ivica Petrovic,Nada Pejnovic,Biljana Ljujic,Sladjana Pavlovic,Marina Miletic Kovacevic,Ilija Jeftic,Aleksandar Djukic,Nevena Draginic,Marijana Andjic,Nebojsa Arsenijevic,Miodrag L Lukic,Nemanja Jovicic

View all publications

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websiteProtocolBooklet

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小鼠Galectin 3 ELISA试剂盒

Mouse Galectin 3 ELISA Kit

ELISA - Mouse Galectin 3 ELISA Kit (AB203369)

Sandwich ELISA - Mouse Galectin 3 ELISA Kit (AB203369)

Sandwich ELISA - Mouse Galectin 3 ELISA Kit (AB203369)

Sandwich ELISA - Mouse Galectin 3 ELISA Kit (AB203369)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台