AB201277

小鼠Eotaxin ELISA试剂盒(CCL11)

Mouse Eotaxin ELISA Kit (CCL11)

Recombinant
SimpleStep
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(2 Publications)

Mouse Eotaxin ELISA Kit (CCL11) is a single-wash 90-min Simplestep used to quantify Mouse Eotaxin (CCL11) with a sensitivity of 8.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Scya11, Ccl11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11

2 Images

Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (AB201277)

sELISA

Supplier Data

Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (AB201277)

Linearity of dilution of the assay. Interpolated concentrations of Eotaxin in mouse serum, plasma (EDTA) and culture media.

The concentrations of Eotaxin were measured in duplicate and interpolated from the Eotaxin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin concentration was determined to be 484 pg/mL in mouse serum and 668 pg/mL in mouse plasma.

sELISA

Supplier Data

Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (AB201277)

Example of Eotaxin standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture supernatant, Serum, EDTA Plasma

反应种属

Mouse

检测类型

Sandwich (quantitative)

灵敏度

= 8.1 pg/mL

范围

23.44 - 1500 pg/mL

检测时间

1h 30m

检测平台

Microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Mouse Eotaxin ELISA Kit (CCL11) (ab201277) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Eotaxin (CCL11) protein in cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Eotaxin (CCL11) with 8.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse Eotaxin (CCL11) is a 8.4 kDa cytokine, constitutively expressed in the thymus and inducibly expressed in the lung, intestine, heart, spleen and kidney. Eotaxin is a chemoattractant for eosinophils during inflammation and allergic reactions, and is also involved in mast cell differentiation and growth of myeloid cell progenitors.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" } ]

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回收率

[ { "sample": "Serum", "range": "84 - 96 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "99 - 107 %", "average": "= 108" }, { "sample": "Cell culture media", "range": "99 - 100 %", "average": "= 99" } ]

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Eotaxin also known as CCL11 or eotaxin-1 is a chemokine belonging to the CC chemokine family. This protein has a molecular mass of approximately 8.4 kDa. Eotaxin is mainly expressed in epithelial cells fibroblasts and smooth muscle cells. High levels of expression occur during inflammatory processes where it acts as a chemoattractant guiding eosinophils to sites of inflammation.

Biological function summary

Eotaxin plays an important role in immune response regulation particularly in allergic reactions. It functions by binding to CCR3 a receptor found on eosinophils therefore facilitating their migration. This process includes involvement in the formation of protein complexes that modulate cell signaling. Eotaxin's primary biological role lies in promoting the accumulation of eosinophils which are important for the immune defense against parasitic infections.

Pathways

Eotaxin participates in the MAPK/ERK signaling pathway which influences cell migration and proliferation. Additionally it operates within the NF-kB pathway often regulating inflammation and immune response. In these pathways eotaxin interacts with other proteins like CCR3 and IL-5 contributing to a coordinated response in immune signaling.

Eotaxin is closely linked to allergic asthma and atopic dermatitis. In both these conditions eotaxin-mediated eosinophil recruitment and activation worsen inflammation. Research shows significant eotaxin expression in asthmatic airway tissues. It also connects with proteins like IL-5 which amplify eosinophilic activity leading to exacerbated allergic responses.

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产品实验方案

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靶点信息

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils (PubMed : 7568052, PubMed : 8574847). Binds to CCR3 (By similarity).

See full target information Ccl11

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Allergologia et immunopathologia 50:75-83 PubMed36086967

2022

Zeaxanthin attenuates OVA-induced allergic asthma in mice by regulating the p38 MAPK/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaosheng Jin,Weijing Jin,Guoping Li,Jisheng Zheng

Scientific reports 7:5334 PubMed28706221

2017

CCL11, a novel mediator of inflammatory bone resorption.

Applications

Unspecified application

Species

Unspecified reactive species

Elin Kindstedt,Cecilia Koskinen Holm,Rima Sulniute,Irene Martinez-Carrasco,Richard Lundmark,Pernilla Lundberg

View all publications

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websiteProtocolBooklet

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小鼠Eotaxin ELISA试剂盒(CCL11)

Mouse Eotaxin ELISA Kit (CCL11)

Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (AB201277)

Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (AB201277)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台