AB234560

小鼠EGF ELISA试剂盒

Mouse EGF ELISA Kit

Recombinant
SimpleStep
了解详情

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(3 Publications)

Mouse EGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse EGF with a sensitivity of 1.03 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Pro-epidermal growth factor, EGF, Egf

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Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

sELISA

Supplier Data

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Interpolated concentrations of spike EGF in mouse serum and plasma samples.

The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean native EGF concentration was determined to be 7.456 pg/mL in neat serum, 63.90 pg/mL in neat plasma (citrate), and not detectable in neat plasma (EDTA). Native data is not shown.

sELISA

Supplier Data

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Interpolated concentrations of native EGF in mouse kidney tissue extract based on a 400 μg/mL extract load.

The concentrations of EGF were measured in duplicate and interpolated from the EGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 90.16 pg/mL in mouse kidney tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Example of mouse EGF standard curve in 1X Cell Extraction Buffer PTR.

The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Example of mouse EGF standard curve in Sample Diluent 25BS.

The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Interpolated concentrations of native EGF in mouse urine and kidney tissue supernatant samples.

The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 10,000 and kidney supernatant 1 : 4. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 600,860 pg/mL in neat urine and 384.1 pg/mL in neat kidney tissue supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Mouse EGF Competitor Std Curve Comparison.

Standard curve comparison between Mouse EGF SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity

sELISA

Supplier Data

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Example of mouse EGF standard curve in Sample Diluent NS.

The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Urine, Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Mouse

检测类型

Sandwich

灵敏度

= 1.03 pg/mL

范围

1.56 - 150 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Mouse EGF ELISA Kit (ab234560) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EGF protein in edta plasma, serum, tissue extracts, urine, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse EGF with 1.03 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]

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回收率

[ { "sample": "Serum", "range": "101 - 105 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "100 - 102 %", "average": "= 101" }, { "sample": "Urine", "range": "92 - 102 %", "average": "= 97" }, { "sample": "Tissue Extracts", "range": "85 - 94 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "101 - 120 %", "average": "= 114" }, { "sample": "Cell culture media", "range": "93 - 101 %", "average": "= 97" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234560-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234560-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234560-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234560-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB234560-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234560-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234560-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234560-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse EGF Lyophilized Recombinant Protein", "number":"AB234560-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse EGF Capture Antibody", "number":"AB234560-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB234560-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB234560-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse EGF Detector Antibody", "number":"AB234560-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB234560-CMP11", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.

Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.

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产品实验方案

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靶点信息

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).

See full target information Egf

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:68 PubMed39994761

2025

Reciprocal regulation of MMP-28 and EGFR is required for sustaining proliferative signaling in PDAC.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtao Hong,Xing Huang,Linghao Xia,Tingbo Liang,Xueli Bai

Nutrients 17: PubMed39861396

2025

Polyphenolic Hispolon Derived from Medicinal Mushrooms of the and Genera Promotes Wound Healing in Hyperglycemia-Induced Impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Shan Liu,Mei-Chou Lai,Yu-Cheng Tzeng,I-Min Liu

Cells 12: PubMed37408251

2023

Immunomodulatory Macrophages Enable E-MNC Therapy for Radiation-Induced Salivary Gland Hypofunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Honma,Takashi I,Makoto Seki,Mayumi Iwatake,Takunori Ogaeri,Kayo Hasegawa,Seigo Ohba,Simon D Tran,Izumi Asahina,Yoshinori Sumita

View all publications

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websiteProtocolBooklet

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小鼠EGF ELISA试剂盒

Mouse EGF ELISA Kit

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

Sandwich ELISA - Mouse EGF ELISA Kit (AB234560)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台