小鼠Cystatin C ELISA试剂盒(ab119590)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 312 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Cystatin C ELISA试剂盒
参阅全部 Cystatin C 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
312 pg/ml - 20000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s mouse Cystatin C in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Cystatin C in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
A Cystatin C specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cystatin C is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Cystatin C amount of sample captured in plate.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-mouse Cystatin C antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- mouse Cystatin C antibody 1 x 100µl Lyophilized recombinant Mouse Cystatin C standard 2 x 20ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
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功能
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. -
组织特异性
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. -
疾病相关
Amyloidosis 6
Macular degeneration, age-related, 11 -
序列相似性
Belongs to the cystatin family. -
翻译后修饰
The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. -
细胞定位
Secreted. - Information by UniProt
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别名
- AD 8
- AD8
- Amyloid angiopathy and cerebral hemorrhage
see all -
数据库链接
- Entrez Gene: 13010 Mouse
- SwissProt: P21460 Mouse
- Unigene: 4263 Mouse
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119590于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab119590.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab119590 被引用在 3 文献中.
- Kleeman SO et al. Cystatin C is glucocorticoid responsive, directs recruitment of Trem2+ macrophages, and predicts failure of cancer immunotherapy. Cell Genom 3:100347 (2023). PubMed: 37601967
- Nair AV et al. Characterizing the interactions of organic nanoparticles with renal epithelial cells in vivo. ACS Nano 9:3641-53 (2015). PubMed: 25790730
- De Chiara L et al. Renal Cells from Spermatogonial Germline Stem Cells Protect against Kidney Injury. J Am Soc Nephrol N/A:N/A (2013). Functional Studies ; Mouse . PubMed: 24136918