小鼠CXCL9 ELISA试剂盒
Mouse CXCL9 ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
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(8 Publications)
Mouse CXCL9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CXCL9 with a sensitivity of 1.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
查看别名
Mig, Scyb9, Cxcl9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Protein m119, Small-inducible cytokine B9, MIG, MuMIG
- ELISA
Supplier Data
ELISA - Mouse CXCL9 ELISA Kit (AB203364)
- sELISA
Supplier Data
Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)
IFN-gamma treatment of Raw 264.7 cell induces secretion of MIG.
Raw 264.7 cells were cultured in the absence or presence of 500 ng/mL mouse recombinant Interferon-gamma protein for 72 hours. The cell culture supernatant samples were collected. The concentrations of MIG were measured in the diluted supernatants in duplicates, interpolated from the MIG standard curve and corrected for sample dilution. Note that 1X Diluted supernatant samples were pre-diluted to 2%. The interpolated, dilution factor-corrected values are plotted in ng of MIG per mL of neat sample (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)
IFN-gamma treatment of Raw 264.7 cell induces expression of MIG.
Raw 264.7 cells were cultured in the absence or presence of 500 ng/mL Mouse recombinant Interferon-gamma protein for 72 hours. The concentrations of MIG were measured in diluted extracts of these cells and of Mouse liver in duplicates, interpolated from the MIG standard curve and corrected for sample dilution. Note that 1X Diluted Raw 264.7 cells extract samples were at 25 μg/mL. Note that 1X Diluted Mouse liver extract samples were at 500 μg/mL. The interpolated, dilution factor-corrected values are plotted in ng of MIG per mg of total protein (mean +/- SD, n=2).
- sELISA
Lab
Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)
Mouse MIG (CXCL9) standard curve comparison data.
Standard curve comparison between mouse MIG (CSCL9) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)
Interpolated concentrations of MIG in mouse serum and plasma samples.
The concentrations of MIG were measured in duplicates, interpolated from the MIG standard curve and corrected for sample dilution. Note that 1X Diluted serum and plasma (citrate and EDTA) samples were pre-diluted to 50%. Note that 1X Diluted plasma (heparin) samples were pre-diluted to 25%. The interpolated, dilution factor-corrected values are plotted in pg of MIG per mL of neat sample (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)
Example of MIG standard curve prepared in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
反应性数据
产品详情
Mouse CXCL9 ELISA Kit ab203364 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse CXCL9 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 1.4 pg/mL.
How the assay works
Mouse CXCL9 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse CXCL9 ELISA Kit ab203364 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture) ab242976
- Anti-CXCL9 antibody [EPR17005-127] - BSA and Azide free (Detector) ab242976
MIG (Monokine Induced by Gamma interferon) is a small cytokine belonging to the CXC motif chemokine family, thus named CXC motif chemokine family ligand 9 (CXCL9). CXCL9 is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. CXCL9 is a T-cell chemoattractant, which is induced by IFN-?.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This cytokine plays a pivotal role in the immune system by regulating leukocyte trafficking. CXCL9 exerts its function through binding to the CXCR3 receptor attracting T cells towards sites of inflammation or infection. This interaction is significant in mediating immune surveillance and host defense. CXCL9 does not form part of a larger protein complex but its chemokine activity is critical for immune system coordination. Scientific studies often highlight its involvement through CXCL9 function and expression analysis.
Pathways
CXCL9 significantly influences the chemokine signaling pathway and the Th1-type adaptive immune response. Within these pathways it interacts closely with other chemokines like CXCL10 and CXCL11 which also bind to the CXCR3 receptor. These pathways highlight the coordinated mobilization of T cells during immune challenges and inflammation emphasizing how CXCL9 is often examined alongside these related chemokines in research settings.
靶点信息
文献 (8)
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