AB100719

小鼠CXCL5 ELISA试剂盒(GCP-2)

Name: 小鼠CXCL5 ELISA试剂盒 (GCP-2) (ab100719)| Abcam中文官网
Brand: Abcam Limited
SKU: AB100719
Price: 7672 CNY
Availability: InStock

Mouse CXCL5 ELISA Kit (GCP-2)

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(5 Publications)

Mouse CXCL5 ELISA Kit (GCP-2) is a Sandwich ELISA for the measurement of Mouse CXCL5 (GCP-2) in Mouse in Biofluids, Cell Culture Media samples.

查看别名

Scyb5, Cxcl5, C-X-C motif chemokine 5, Cytokine LIX, Small-inducible cytokine B5

1 Images

Sandwich ELISA - Mouse CXCL5 ELISA Kit (GCP-2) (AB100719)

sELISA

Unknown

Sandwich ELISA - Mouse CXCL5 ELISA Kit (GCP-2) (AB100719)

Representative standard curve using ab100719

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Mouse

检测类型

Sandwich

灵敏度

< 35 pg/mL

范围

31.25 - 2000 pg/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Abcam's CXCL5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CXCL5 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for CXCL5 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CXCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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回收率

[ { "sample": "Cell culture supernatant", "range": "74 - 94 %", "average": "= 83.94" }, { "sample": "Serum", "range": "86 - 106 %", "average": "= 98.05" }, { "sample": "Plasma", "range": "83 - 102 %", "average": "= 93.11" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100719-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100719-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100719-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CXCL5 Microplate (12 x 8 wells)", "number":"AB100719-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100719-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100719-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse CXCL5", "number":"AB100719-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse CXCL5 Standard (lyophilized)", "number":"AB100719-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100719-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100719-CMP02", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CXCL5 also known as LIX (Liposaccharide-induced CXC chemokine) is a small cytokine belonging to the CXC chemokine family. It plays a significant role in immunological responses by acting as a chemoattractant for neutrophils. CXCL5 has a molecular mass of around 12 kDa. The protein is mainly expressed in neutrophils epithelial cells and endothelial cells. It is often studied alongside its competitors in different therapeutic and diagnostic contexts.

Biological function summary

CXCL5 affects inflammation and the immune response by modulating neutrophil recruitment to sites of infection or injury. It is a part of the larger network of chemokines that direct leukocyte movement throughout the body. While CXCL5 does not form a complex in a traditional sense it does interact dynamically with CXCR2 receptor to exert its effects. These interactions are pivotal to the protein’s role in orchestrating the immune system’s response to diverse stimuli.

Pathways

CXCL5 plays a part in the chemokine signaling pathway and the inflammatory response pathway. These pathways are important in mediating immune surveillance and inflammation. CXCL5 through these pathways has a strong connection to other CXC family proteins like CXCL1 and the receptor CXCR2 which allows for a coordinated response during immune challenges and recovery processes.

CXCL5 is relevant in the context of chronic inflammatory disorders such as rheumatoid arthritis and cancer specifically its role in tumor-related inflammation. In these conditions CXCL5 is linked to the exacerbation of inflammatory states and promotion of tumor growth via angiogenesis. The protein's interactions with CXCR2 play a significant role in these disease processes representing potential therapeutic targets for managing inflammation and modulating cancer progression.

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产品实验方案

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靶点信息

May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation.

See full target information Cxcl5

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Experimental hematology & oncology 13:11 PubMed38291516

2024

Targeting focal adhesion kinase boosts immune response in KRAS/LKB1 co-mutated lung adenocarcinoma via remodeling the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Qiao,Fei Zhou,Xinyu Liu,Tao Jiang,Haowei Wang,Xuefei Li,Chao Zhao,Lei Cheng,Xiaoxia Chen,Shengxiang Ren,Zaiqi Wang,Caicun Zhou

Oncoimmunology 11:2059876 PubMed35402081

2022

CXCL5-mediated accumulation of mature neutrophils in lung cancer tissues impairs the differentiation program of anticancer CD8 T cells and limits the efficacy of checkpoint inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Simoncello,Giulia Maria Piperno,Nicoletta Caronni,Roberto Amadio,Ambra Cappelletto,Giulia Canarutto,Silvano Piazza,Silvio Bicciato,Federica Benvenuti

Molecular metabolism 34:112-123 PubMed32180551

2020

Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Minxuan Xu,Chenxu Ge,Yuting Qin,Deshuai Lou,Qiang Li,Jing Feng,Yekuan Wu,Linfeng Hu,Bochu Wang,Jun Tan

Nature communications 5:3056 PubMed24445999

2014

A dual role for autophagy in a murine model of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuan Rao,Luigi Tortola,Thomas Perlot,Gerald Wirnsberger,Maria Novatchkova,Roberto Nitsch,Peter Sykacek,Lukas Frank,Daniel Schramek,Vukoslav Komnenovic,Verena Sigl,Karin Aumayr,Gerald Schmauss,Nicole Fellner,Stephan Handschuh,Martin Glösmann,Pawel Pasierbek,Michaela Schlederer,Guenter P Resch,Yuting Ma,Heng Yang,Helmuth Popper,Lukas Kenner,Guido Kroemer,Josef M Penninger

Journal of immunology (Baltimore, Md. : 1950) 189:5139-46 PubMed23109722

2012

Glutamine suppresses airway neutrophilia by blocking cytosolic phospholipase A(2) via an induction of MAPK phosphatase-1.

Applications

Unspecified application

Species

Mouse

Chang-Hoon Lee,Hae-Kyoung Kim,June-Mo Kim,Otgonzaya Ayush,Suhn-Young Im,Dae-Kyu Oh,Hern-Ku Lee

View all publications

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websiteProtocolBooklet

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小鼠CXCL5 ELISA试剂盒(GCP-2)

Mouse CXCL5 ELISA Kit (GCP-2)

Sandwich ELISA - Mouse CXCL5 ELISA Kit (GCP-2) (AB100719)