AB264609

小鼠Complement C5 ELISA试剂盒

Mouse Complement C5 ELISA Kit

Recombinant
SimpleStep
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(5 Publications)

Mouse Complement C5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Complement C5 with a sensitivity of 1.97 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Hc, C5, Complement C5, Hemolytic complement

3 Images

ELISA - Mouse Complement C5 ELISA Kit (AB264609)

ELISA

Supplier Data

ELISA - Mouse Complement C5 ELISA Kit (AB264609)

sELISA

Supplier Data

Sandwich ELISA - Mouse Complement C5 ELISA Kit (AB264609)

Interpolated concentrations of native Complement C5 in mouse serum and plasma samples.

The concentrations of Complement C5 were measured in duplicates, interpolated from the Complement C5 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 3%, plasma (EDTA) 0.75%, and plasma (heparin) 0.38%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C5 concentration was determined to be 4.06 μg/mL in serum, 5.61 μg /mL in plasma (EDTA) and 4.40 μg /mL in plasma (Heparin).

sELISA

Supplier Data

Sandwich ELISA - Mouse Complement C5 ELISA Kit (AB264609)

Example of mouse Complement C5 standard curve in Sample Diluent NS + 1X Enhancer.

The Complement C5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

样品类型

Cell culture media, EDTA Plasma, Heparin Plasma, Serum

反应种属

Mouse

检测类型

Sandwich

灵敏度

= 1.97 ng/mL

范围

4.69 - 300 ng/mL

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Mouse Complement C5 ELISA Kit ab264609 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Complement C5 in cell culture media, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.97 ng/mL.

How the assay works

Mouse Complement C5 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Complement C5 ELISA Kit ab264609 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-C5 antibody [EPR21072-146] - BSA and Azide free (Capture) ab267431
- Anti-C5 antibody [EPR21072-119] - BSA and Azide free (Detector) ab267432

Mouse Complement C5 activation by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. Mouse Complement C5 shares a 64.4%, and 81.8% sequence homology with rat and human, respectively.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Protein", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]

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回收率

[ { "sample": "Cell culture media", "range": "110 - 114 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "90 - 101 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "98 - 103 %", "average": "= 100" }, { "sample": "Serum", "range": "105 - 117 %", "average": "= 109" } ]

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

C5 also known as complement 5 is a protein with a mass of approximately 190 kDa. This protein plays a mechanical role as part of the complement system within the immune response. C5 is expressed in the bloodstream and is produced mainly by the liver. Upon activation it splits into C5a and C5b where C5a acts as a potent anaphylatoxin while C5b initiates the assembly of the membrane attack complex. Due to its important functions the production and regulation of C5 are tightly controlled within the body.

Biological function summary

The activation of this target contributes significantly to inflammatory processes and host defense. It is part of the larger complement system where it aligns with other complement proteins like C3 and forms a pathway important for immune system functioning. C5 is integral to the terminal complement complex formation which results in the lysis of target cells. The cleavage product C5a has near-instant effects as a chemotactic agent recruiting other immune cells to sites of infection.

Pathways

The protein has a fundamental position in the classical and alternative complement pathways. These pathways converge at the level of C5 where its cleavage is necessary for further downstream effects. C5 interacts extensively with C3 in these pathways highlighting their joint role in amplifying the immune response. The membrane attack complex which C5 helps initiate is a vital endpoint in these pathways promoting the destruction of pathogen cell membranes.

C5 has been linked to conditions like hereditary angioedema and atypical hemolytic uremic syndrome. Dysregulation of C5 or its pathways can lead to chronic inflammation excessive tissue damage or inefficient pathogen clearance. In such conditions related proteins like C3 also show altered activity patterns. Targeting C5 with specific antibodies has emerged as a therapeutic strategy to manage these diseases aiming to mitigate the pathological activation of the complement system.

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产品实验方案

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靶点信息

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.. C5a anaphylatoxin. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

See full target information C5

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Acta biomaterialia 179:83-94 PubMed38447809

2024

Active immunotherapy for C5a-mediated inflammation using adjuvant-free self-assembled peptide nanofibers.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly M Hainline,Helena Freire Haddad,Anna Gilpin,Elizabeth J Curvino,Shyni Varghese,Joel H Collier

Cell 187:897-913.e18 PubMed38280374

2024

Gut complement induced by the microbiota combats pathogens and spares commensals.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wu,Wen Zheng,Xinyang Song,Bin Bao,Yuanyou Wang,Deepshika Ramanan,Daping Yang,Rui Liu,John C Macbeth,Elyza A Do,Warrison A Andrade,Tiandi Yang,Hyoung-Soo Cho,Francesca S Gazzaniga,Marit Ilves,Daniela Coronado,Charlotte Thompson,Saiyu Hang,Isaac M Chiu,Jeffrey R Moffitt,Ansel Hsiao,John J Mekalanos,Christophe Benoist,Dennis L Kasper

The EMBO journal 42:e113975 PubMed37718683

2023

Infection and inflammation stimulate expansion of a CD74 Paneth cell subset to regulate disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Iyshwarya Balasubramanian,Sheila Bandyopadhyay,Juan Flores,Jared Bianchi-Smak,Xiang Lin,Haoran Liu,Shengxiang Sun,Natasha B Golovchenko,Yue Liu,Dahui Wang,Radha Patel,Ivor Joseph,Panan Suntornsaratoon,Justin Vargas,Peter Hr Green,Govind Bhagat,Stephen M Lagana,Wang Ying,Yi Zhang,Zhihan Wang,Wei Vivian Li,Sukhwinder Singh,Zhongren Zhou,George Kollias,Laura A Farr,Shannon N Moonah,Shiyan Yu,Zhi Wei,Edward M Bonder,Lanjing Zhang,Pawel R Kiela,Karen L Edelblum,Ronaldo Ferraris,Ta-Chiang Liu,Nan Gao

JCI insight 8: PubMed36809299

2023

Mechanism and intervention of murine transfusion-related acute lung injury caused by anti-CD36 antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Da-Wei Chen,Tian Kang,Xiu-Zhang Xu,Wen-Jie Xia,Xin Ye,Yong-Bin Wu,Yao-Ri Xu,Jing Liu,Hui Ren,Jing Deng,Yang-Kai Chen,Hao-Qiang Ding,Muhammad Aslam,Wioleta M Zelek,B Paul Morgan,Rick Kapur,Sentot Santoso,Yong-Shui Fu

Cell death & disease 13:975 PubMed36402750

2022

Inhibition of the immunoproteasome modulates innate immunity to ameliorate muscle pathology of dysferlin-deficient BlAJ mice.

Applications

Unspecified application

Species

Unspecified reactive species

A Farini,L Tripodi,C Villa,F Napolitano,F Strati,D Molinaro,F Facciotti,B Cassani,Y Torrente

View all publications

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websiteProtocolBooklet

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小鼠Complement C5 ELISA试剂盒

Mouse Complement C5 ELISA Kit

ELISA - Mouse Complement C5 ELISA Kit (AB264609)

Sandwich ELISA - Mouse Complement C5 ELISA Kit (AB264609)

Sandwich ELISA - Mouse Complement C5 ELISA Kit (AB264609)