AB100674

小鼠CD40 ELISA试剂盒

Name: 小鼠CD40 ELISA试剂盒 (ab100674)| Abcam中文官网
Brand: Abcam Limited
SKU: AB100674
Price: 7672 CNY
Availability: InStock
Rating: 4 (2 reviews)

Mouse CD40 ELISA Kit

(2 Reviews)

(4 Publications)

Mouse CD40 ELISA Kit is a Sandwich ELISA for the measurement of Mouse CD40 in Mouse in Biofluids, Cell Culture Media samples.

查看别名

CD40, Tnfrsf5, Cd40, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor

2 Images

Sandwich ELISA - Mouse CD40 ELISA Kit (AB100674)

sELISA

Supplier Data

Sandwich ELISA - Mouse CD40 ELISA Kit (AB100674)

Representative Standard Curve using ab100674

sELISA

Supplier Data

Sandwich ELISA - Mouse CD40 ELISA Kit (AB100674)

Representative Standard Curve using ab100674

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Mouse

检测类型

Sandwich

灵敏度

< 3 pg/mL

范围

2.06 - 1500 pg/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Abcam's CD40 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD40 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse CD40 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Mouse CD40 ELISA Kit (TNFRS5) (ab206307) from our SimpleStep ELISA^® range.

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回收率

[ { "sample": "Cell culture supernatant", "range": "74 - 93 %", "average": "= 84.23" }, { "sample": "Serum", "range": "71 - 87 %", "average": "= 77.26" }, { "sample": "Plasma", "range": "69 - 87 %", "average": "= 76.68" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100674-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100674-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100674-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100674-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100674-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CD40 Microplate (12 x 8 wells)", "number":"AB100674-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse CD40", "number":"AB100674-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse CD40 Standard (lyophilized)", "number":"AB100674-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100674-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100674-CMP02", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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产品实验方案

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靶点信息

Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed : 12881420).

See full target information Cd40

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 11:17262 PubMed34446808

2021

Downregulation of CD40L-CD40 attenuates seizure susceptibility and severity of seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Pototskiy,Katherine Vinokuroff,Andrew Ojeda,C Kendall Major,Deepak Sharma,Taylor Anderson,Kendall Howard,Ronen Borenstein,Alberto E Musto

Experimental and therapeutic medicine 16:3261-3267 PubMed30233673

2018

Regulatory role of microRNA-185 in the recovery process after ankle fracture.

Applications

Unspecified application

Species

Unspecified reactive species

Deping Sun,Juntao Liu,Qingpeng Shi,Haibo Mu,Dongsheng Zhou

Experimental and therapeutic medicine 15:4550-4556 PubMed29849780

2018

Aberrant expression of miR-663 and transforming growth factor-β1 in nasal polyposis in children.

Applications

Unspecified application

Species

Unspecified reactive species

Hailing Yu,Jianbao Ju,Jingdong Liu,Da Li

Experimental and therapeutic medicine 13:1127-1132 PubMed28450952

2017

MicroRNA-185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor-β1.

Applications

ELISA

Species

Human

Pengfei Zhao,Shaochun Wang,Yingjie Zhou,Huailiang Zheng,Gang Zhao

View all publications

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websiteProtocolBooklet

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

小鼠CD40 ELISA试剂盒

Mouse CD40 ELISA Kit

Sandwich ELISA - Mouse CD40 ELISA Kit (AB100674)

Sandwich ELISA - Mouse CD40 ELISA Kit (AB100674)