小鼠CCL27 ELISA试剂盒(CTACK)
Mouse CCL27 ELISA Kit (CTACK)
- Recombinant
- SimpleStep
- 了解详情
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Mouse CCL27 ELISA Kit (CTACK) is a single-wash 90-min Simplestep used to quantify Mouse CCL27 (CTACK) with a sensitivity of 9.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Ilc, Scya27, Ccl27, C-C motif chemokine 27, CC chemokine ILC, Cutaneous T-cell-attracting chemokine, ESkine, IL-11 R-alpha-locus chemokine, Skinkine, Small-inducible cytokine A27, CTACK, ALP, mILC
- sELISA
Supplier Data
Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (AB202406)
CCL27 levels in stimulated (1.5% PHA + 10 ng/mL PMA, 72 hrs (+)) and unstimulated (-) mouse L929 supernatants.
A blank cell culture media control was also analyzed, and was negative. The 1X dilution was 100% for both the stimulated and unstimulated supernatants. Interpolated data values (mean +/- SD, n = 2) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (AB202406)
Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.
The 1X dilution was 3.13% for mouse serum, 6.25% for citrate and EDTA plasma, and 1.56% for heparin plasma. Interpolated data values (mean +/- SD, n = 2) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse CCL27 ELISA Kit (CTACK) (AB202406)
Example of CCL27 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
搭配使用产品
反应性数据
产品详情
Mouse CCL27 ELISA Kit (CTACK) (ab202406) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL27 (CTACK) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL27 (CTACK) with 9.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CCL27 is a chemotactic factor that attracts skin-associated memory T-lymphocytes, and binds to CCR10. It may play a role in cell migration during embryogenesis, and may mediate lymphocyte homing to cutaneous sites. Nuclear forms of CCL27 may facilitate cellular migration by inducing cytoskeletal relaxation. There is evidence that CCL27 is strongly upregulated in atopic dermatitis, contact dermatitis, and psoriasis skin lesions.
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规格
性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CCL27 attracts T cells to the skin's surface facilitating immune responses. Although not part of a larger complex CCL27 interacts with its receptor CCR10 on T cells directing them to skin sites where they can perform immune surveillance and respond to pathogens. This chemokine-receptor interaction is essential for maintaining skin homeostasis and effective immune responses.
Pathways
The role of CCL27 gets significant attention in the CCR10-CTACK axis which is pivotal in the cutaneous immune system's functioning. Additionally the CCL27/CCR10 pathway has connections to the wider chemokine signaling network that regulates leukocyte trafficking. Through these associations CCL27 is connected to other chemokines and receptors including CCL28 which also shares CCR10 as a receptor highlighting the integrated nature of chemokine function in immune pathways.
靶点信息
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