小鼠Cathepsin B ELISA试剂盒(ab119585)
Key features and details
- Sensitivity: 5 pg/well
- Range: 156 pg/well - 10000 pg/well
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
-
产品名称
小鼠Cathepsin B ELISA试剂盒
参阅全部 Cathepsin B 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 242pg/ml 14.76 6.1% 2 16 1828pg/ml 100.54 5.5% 3 16 4749pg/ml 370.42 7.8% 批次间 样品 n Mean SD CV% 1 24 229pg/ml 13.96 6.1% 2 24 1916pg/ml 128.37 6.7% 3 24 4534pg/ml 439.79 9.7% -
样品类型
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 5 pg/well -
范围
156 pg/well - 10000 pg/well -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse
不与反应: Human -
产品概述
Abcam’s Mouse Cathepsin B in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse Cathepsin B in cell culture supernatants, serum and plasma (heparin, EDTA).
A Cathepsin B specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin B is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Cathepsin B amount of sample captured in plate.
-
经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-mouse Cathepsin B antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-mouse Cathepsin B antibody 1 x 100µl Lyophilized recombinant mouse Cathepsin B standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
-
功能
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
序列相似性
Belongs to the peptidase C1 family. -
细胞定位
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
别名
- Amyloid precursor protein secretase
- APP secretase
- APPS
see all -
数据库链接
- Entrez Gene: 13030 Mouse
- SwissProt: P10605 Mouse
- Unigene: 236553 Mouse
- Unigene: 472420 Mouse
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119585于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
-
Typical Standard Curve
Representative Standard Curve Using ab119585.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab119585 被引用在 3 文献中.
- Raas Q et al. Peroxisomal defects in microglial cells induce a disease-associated microglial signature. Front Mol Neurosci 16:1170313 (2023). PubMed: 37138705
- Liu J et al. DCN released from ferroptotic cells ignites AGER-dependent immune responses. Autophagy 18:2036-2049 (2022). PubMed: 34964698
- Mees LM et al. Low-Intensity Exercise in Mice Is Sufficient to Protect Retinal Function During Light-Induced Retinal Degeneration. Invest Ophthalmol Vis Sci 60:1328-1335 (2019). PubMed: 30933260