AB263884

小鼠C3 ELISA试剂盒

Mouse C3 ELISA Kit

Recombinant
SimpleStep
了解详情

Be the first to review this product! Submit a review

(6 Publications)

Mouse C3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse C3 with a sensitivity of 25.52 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Complement C3, HSE-MSF, C3

5 Images

ELISA

Supplier Data

ELISA - Mouse C3 ELISA Kit (AB263884)

sELISA

Supplier Data

Sandwich ELISA - Mouse C3 ELISA Kit (AB263884)

Interpolated concentrations of native C3 in mouse kidney and liver tissue extracts based on a 10 µg/mL extract load respectively.

The concentrations of C3 were measured in duplicate and interpolated from the C3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C3 concentration was determined to be 3,036 pg/mL in kidney tissue and 2,949 pg/mL in liver tissue.

sELISA

Supplier Data

Sandwich ELISA - Mouse C3 ELISA Kit (AB263884)

Interpolated concentrations of native C3 in mouse serum and plasma samples.

The concentrations of C3 were measured in duplicates, interpolated from the C3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 133,333, plasma (citrate) 1 : 100,000, plasma (EDTA) 1 : 100,000, and plasma (heparin) 1 : 133,333. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C3 concentration was determined to be 172.6 μg/mL in serum, 117.6 μg/mL in plasma (citrate), 234.6 μg/mL in plasma (EDTA), and 410.8 μg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Mouse C3 ELISA Kit (AB263884)

Example of mouse C3 standard curve in Sample Diluent NS + Enhancer.

The C3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse C3 ELISA Kit (AB263884)

Interpolated concentrations of native C3 in mouse tissue culture supernatant sample.

The concentrations of C3 were measured in duplicates, interpolated from the C3 standard curves and corrected for sample dilution. Undiluted samples are as follows : liver supernatant 1 : 80. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C3 concentration was determined to be 207.0 ng/mL liver supernatant.

关键信息

检测方法

Colorimetric

样品类型

Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Mouse

检测类型

Sandwich

灵敏度

= 25.52 pg/mL

范围

62.5 - 4000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

产品详情

Mouse C3 ELISA Kit ab263884 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse C3 in cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 25.52 pg/mL.

How the assay works

Mouse C3 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse C3 ELISA Kit ab263884 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-C3 antibody [EPR22398-156] - BSA and Azide free (Capture) ab259699
- Anti-C3 antibody [EPR22398-105] - BSA and Azide free (Detector) ab259700

C3, often known as complement component 3, is a protein part of the immune system where it plays a central role in the activation of the complement system. C3 is mainly synthesized in the liver and found to be the most abundant complement protein in serum. C3 is comprised of alpha and beta chains connected covalently by a single disulfide bond and associated by non-covalent forces. Studies have shown people with C3 deficiency are susceptible to bacterial infection. Standard protein in this product is the C3c alpha chain fragment 2.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]

style

left-column

回收率

[ { "sample": "Cell culture supernatant", "range": "108 - 112 %", "average": "= 110" }, { "sample": "Serum", "range": "107 - 113 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "101 - 119 %", "average": "= 112" }, { "sample": "Cell culture media", "range": "94 - 96 %", "average": "= 95" }, { "sample": "Tissue Extracts", "range": "106 - 115 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "94 - 116 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "106 - 114 %", "average": "= 111" } ]

style

left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab263884-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB263884-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB263884-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB263884-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB263884-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB263884-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB263884-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB263884-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse C3 Capture Antibody", "number":"AB263884-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB263884-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse C3 Lyophilized Recombinant Protein", "number":"AB263884-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB263884-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse C3 Detector Antibody", "number":"AB263884-CMP10", "productcode":"" } ] } } }

style

left-column

性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Complement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.

Biological function summary

Complement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.

Pathways

The complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.

Complement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.

style

left-column

产品实验方案

style

left-column

靶点信息

Precursor of non-enzymatic components of the classical, alternative, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.. Complement C3b. Non-enzymatic component of C5 convertase. Generated following cleavage by C3 convertase, it covalently attaches to the surface of pathogens, where it acts as an opsonin that marks the surface of antigens for removal. Complement C3b binds covalently via its reactive thioester, to cell surface carbohydrates or immune aggregates. Together with complement C4b, it then recruits the serine protease complement C2b to form the C5 convertase, which cleaves and activate C5, the next component of the complement pathways. In the alternative complement pathway, recruits the serine protease CFB to form the C5 convertase that cleaves and activates C5.. C3a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C3 convertase. Acts by binding to its receptor, C3AR1, activating G protein-coupled receptor signaling, promoting the phosphorylation, ARRB2-mediated internalization and endocytosis of C3AR1. C3a anaphylatoxin stimulates the activation of immune cells such as mast cells and basophilic leukocytes to release inflammation agents, such as cytokines, chemokines and histamine, which promote inflammation development. Also acts as potent chemoattractant for the migration of macrophages and neutrophils to the inflamed tissues, resulting in neutralization of the inflammatory triggers by multiple ways, such as phagocytosis and generation of reactive oxidants.. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial triglyceride clearance. Appears to stimulate triglyceride synthesis via activation of the PLC, MAPK and AKT signaling pathways. Acts by binding to its receptor, C5AR2, activating G protein-coupled receptor signaling, promoting the phosphorylation, ARRB2-mediated internalization and endocytosis of C5AR2. In contrast to C3a anaphylatoxin peptide, does not show pro-inflammatory activity.. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.

See full target information C3

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 99:e0180424 PubMed39846741

2025

Mitochondrial injury and complement dysregulation are drivers of pathological inflammation in viral myocarditis.

Applications

Unspecified application

Species

Unspecified reactive species

Yasir Mohamud,Amirhossein Bahreyni,Sinwoo Wendy Hwang,Jingfei Carly Lin,Zhihan Claire Wang,Jingchun Zhang,Honglin Luo

Endocrinology 165: PubMed38123514

2023

Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Abraham Behrmann,Dalian Zhong,Li Li,Shangkui Xie,Megan Mead,Parastoo Sabaeifard,Mohammad Goodarzi,Andrew Lemoff,Julia Kozlitina,Dwight A Towler

Cellular & molecular biology letters 28:79 PubMed37828427

2023

Activation of BZW1 by CEBPB in macrophages promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress in MRL/lpr lupus-prone mice.

Applications

Unspecified application

Species

Unspecified reactive species

Huimeng Qi,Zhaoguo Zheng,Qiang Liu

Immunity 56:1809-1824.e10 PubMed37499656

2023

Cell-autonomous regulation of complement C3 by factor H limits macrophage efferocytosis and exacerbates atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Máté G Kiss,Nikolina Papac-Miličević,Florentina Porsch,Dimitrios Tsiantoulas,Tim Hendrikx,Minoru Takaoka,Huy Q Dinh,Marie-Sophie Narzt,Laura Göderle,Mária Ozsvár-Kozma,Michael Schuster,Nikolaus Fortelny,Anastasiya Hladik,Sylvia Knapp,Florian Gruber,Matthew C Pickering,Christoph Bock,Filip K Swirski,Klaus Ley,Alma Zernecke,Clément Cochain,Claudia Kemper,Ziad Mallat,Christoph J Binder

Journal of virology 97:e0055623 PubMed37191498

2023

Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Gi Uk Jeong,Sumin Lee,Do Yeon Kim,Jaemyun Lyu,Gun Young Yoon,Kyun-Do Kim,Keun Bon Ku,Junsu Ko,Young-Chan Kwon

Toxicological research 39:251-262 PubMed37008699

2023

Characterization of complement C3 as a marker of alpha-amanitin toxicity by comparative secretome profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Doeun Kim,Min Seo Lee,Hyunchae Sim,Sangkyu Lee,Hye Suk Lee

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

小鼠C3 ELISA试剂盒

Mouse C3 ELISA Kit

ELISA - Mouse C3 ELISA Kit (AB263884)

Sandwich ELISA - Mouse C3 ELISA Kit (AB263884)

Sandwich ELISA - Mouse C3 ELISA Kit (AB263884)

Sandwich ELISA - Mouse C3 ELISA Kit (AB263884)

Sandwich ELISA - Mouse C3 ELISA Kit (AB263884)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台