小鼠c-Kit ELISA试剂盒(CD117) (ab203361)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 30 pg/ml
- Range: 0.23 ng/ml - 15 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠c-Kit ELISA试剂盒(CD117)
参阅全部 c-Kit 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5 2.6% 批次间 样品 n Mean SD CV% Overall 3 5.4% -
样品类型
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
30 pg/ml -
范围
0.23 ng/ml - 15 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 94 92% - 94% Serum 118 115% - 120% Hep Plasma 109 104% - 114% EDTA Plasma 105 93% - 144% Cit plasma 110 103% - 119% Mouse brain extract 104 103% - 106% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse
不与反应: Sheep, Goat, Cow, Pig -
产品概述
Mouse c-Kit ELISA Kit (CD117) (ab203361) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of c-Kit (CD117) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse c-Kit (CD117) with 30 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
c-Kit (CD117) is a member of the type III subfamily of receptor tyrosine kinases and is the cellular receptor for stem cell factor. Kit is essential for cell survival and proliferation, stem cell maintenance, mast cell development, melanogenesis, cellular migration, hematopoiesis, and gametogenesis. Mutations in human c-Kit are associated with gastrointestional stromal tumors, piebaldism, acute myelogenous leukemia, and mast cell disease.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse c-Kit Capture Antibody 1 x 600µl 10X Mouse c-Kit Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI2 1 x 6ml Mouse C-Kit Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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相关性
KIT encodes the human homolog of the proto-oncogene c-kit. c-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. -
细胞定位
Cell Membrane; single-pass type I membrane protein -
别名
- CD117
- Mast/stem cell growth factor receptor precursor
- PBT
see all -
数据库链接
- Entrez Gene: 16590 Mouse
- SwissProt: Q7TS86 Mouse
- SwissProt: Q6QJB8 Mouse
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab203361于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of c-KIT standard curve prepared in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
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Example of c-KIT standard curve prepared in 1X Cell Extraction buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of c-Kit in mouse plasma (EDTA, heparin and citrate).The concentrations of c-Kit were measured in duplicate and interpolated from the c-Kit standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of c-Kit in mouse brain and lung extract.The concentrations of c-Kit were measured in duplicate and interpolated from the c-Kit standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Interpolated values of c-Kit are plotted based on an extract load of 200 µg/mL.
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Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (ab203361)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (ab203361)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (ab203361)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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