小鼠Beta-2-Microglobulin ELISA试剂盒(ab223590)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.165 pg/ml
- Range: 3.125 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Beta-2-Microglobulin ELISA试剂盒
参阅全部 beta 2 Microglobulin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 5 2.8% 批次间 样品 n Mean SD CV% Serum 3 3.2% -
样品类型
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1.165 pg/ml -
范围
3.125 pg/ml - 200 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 114 110% - 118% Urine 98 95% - 100% Serum 102 97% - 106% Hep Plasma 93 82% - 103% EDTA Plasma 103 101% - 107% Cit plasma 102 101% - 103% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse -
产品概述
Mouse Beta-2-Microglobulin ELISA Kit (ab223590) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Beta-2-Microglobulin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Beta-2-Microglobulin with 1.165 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Beta-2-Microglobulin is the light chain of the class I major histocompatibility complex (MHC); it forms heterodimers with classical (HLA-A, -B, -C) and non-classical (HLA-E, -F, -G) class I MHC heavy chains and with class I non-MHC heavy chains (CD1). Beta-2-Microglobulin is involved in the presentation of peptide antigens to the immune system. Beta-2-Microglobulin can dissociate from the MHC and circulate in complex with alpha 2-Macroglobulin.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 384 tests 10X Mouse Beta-2-Microglobulin Capture Antibody 1 x 600µl 1 x 600µl 10X Mouse Beta-2-Microglobulin Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 6 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml 1 x 50ml Antibody Diluent 5BR 1 x 6ml 1 x 6ml Denaturant 1 x 500µl 4 x 500µl Mouse Beta-2-Microglobulin Lyophilized Recombinant Protein 2 vials 2 vials Plate Seal 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit Stop Solution 1 x 12ml 2 x 12ml TMB Development Solution 1 x 12ml 2 x 12ml -
研究领域
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功能
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
疾病相关
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
序列相似性
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
翻译后修饰
Glycation of Ile-21 is observed in long-term hemodialysis patients. -
细胞定位
Secreted. Detected in serum and urine. - Information by UniProt
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别名
- B2M
- B2MG_HUMAN
- Beta 2 microglobin
see all -
数据库链接
- Entrez Gene: 12010 Mouse
- SwissProt: P01887 Mouse
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223590于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (ab223590)Interpolated concentration of native Beta-2-Microglobulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:100,000 mouse serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
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Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (ab223590)Example of mouse Beta-2-Microglobulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed. -
Example of mouse Beta-2-Microglobulin standard curve in Sample Diluent NS + EnhancerBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Beta-2-Microglobulin in mouse serum, and plasma samplesThe concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:100,000, plasma (citrate) 1:100,000, plasma (heparin) 1:100,000, and plasma (EDTA) 1:100,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 19.48 µg/mL in neat serum, 15.15 µg/mL in neat plasma (citrate), 18.74 µg/mL in neat plasma (heparin), and 15.87 µg/mL in neat plasma (EDTA).
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Interpolated concentrations of native Beta-2-Microglobulin in mouse urine and lung tissue culture supernatant samplesThe concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:100, lung supernatant 1:5,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 16.12 ng/mL in neat urine, 440.7 ng/mL in neat lung tissue culture supernatant.
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Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (ab223590)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (ab223590)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (ab223590)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab223590 被引用在 1 文献中.
- Sun L et al. Isoliquiritigenin attenuates acute renal injury through suppressing oxidative stress, fibrosis and JAK2/STAT3 pathway in streptozotocin-induced diabetic rats. Bioengineered 12:11188-11200 (2021). PubMed: 34784849