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AB285274

小鼠Alkaline Phosphatase ELISA试剂盒

Mouse Alkaline Phosphatase ELISA Kit

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(7 Publications)

Mouse Alkaline Phosphatase ELISA Kit is a Sandwich ELISA for the measurement of Mouse Alkaline Phosphatase in Mouse in Biofluids, Cell Culture Media samples.

查看别名

Akp-3, Akp3, Iap, Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, Alkaline phosphatase 3

1 Images
Sandwich ELISA - Mouse Alkaline Phosphatase ELISA Kit (AB285274)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Alkaline Phosphatase ELISA Kit (AB285274)

ALP Standard Curve.

Standard curve anaylsis. For reference use only.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Mouse

检测类型

Sandwich

Quantitative
灵敏度

< 18.75 pg/mL

范围

31.25 - 2000 pg/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

Assay Kits

AB282882

Mouse ALT ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

  • 5
  • 1
Assay Kits

AB263882

Mouse AST ELISA Kit (Aspartate Aminotransferase)

Recombinant|SimpleStep

ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

  • 5
  • 1
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Assay Kits

AB83369

Alkaline Phosphatase Assay Kit (Colorimetric)

Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

The Mouse ALP ELISA kit (ab285274, E4573) is a sandwich ELISA assay for the quantitative measurement of ALP in mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of ALP captured from the samples.
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精确度

[ { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" }, { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alkaline Phosphatase also known as ALP or ALKPHOS is an enzyme that removes phosphate groups from various molecules like nucleotides and proteins. This enzymatic activity requires alkaline conditions with a pH optimum around 8–10. ALP is anchored to the cell membrane and exists in several isoforms each with a mass around 57-70 kDa. Different tissues express specific isoforms such as bone liver kidney and placenta. Osteoblasts for example show high levels of alkaline phosphatase expression often detected by techniques like ALP staining or alkaline phosphatase staining.
Biological function summary

Alkaline phosphatase plays important roles in dephosphorylation processes. Particularly in bone it is associated with osteoblast activity where it is important for bone mineralization. The enzyme is not known to be part of a large protein complex but its activity aids in the conversion of inorganic pyrophosphate into phosphate which promotes the deposition of calcium phosphate in bone. ALP staining and ALP staining osteoblast can be used in laboratory settings for studying the mineralization and function of osteoblasts.

Pathways

Alkaline phosphatase is key in the regulation of the phosphate group levels within the organism particularly in the bone mineralization process. It participates in the vitamin D metabolic pathway which influences calcium and phosphorus homeostasis. Its function has a dynamic relationship with proteins like NPP1 which regulates levels of inorganic pyrophosphate acting counter to alkaline phosphatase's phosphate-producing activity.

Defects in alkaline phosphatase activity are linked to conditions like hypophosphatasia and hyperphosphatasia where mineralization processes are disrupted. In hypophosphatasia decreased ALP activity leads to improper bone formation due to accumulation of inorganic pyrophosphate. This interaction is notable alongside proteins such as tissular nonspecific alkaline phosphatase. On the other hand elevated ALP levels often indicate liver or bone disease pathology where increased enzyme levels reflect altered tissue turnover.
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产品实验方案

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靶点信息

Alkaline phosphatase that can hydrolyze various phosphate compounds.
See full target information Iap
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 9: PubMed40536537

2025

Rbpj deletion in hepatic progenitor cells attenuates endothelial responses and fibrosis in DDC-fed mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sanghoon Lee,Lu Ren,Weiwei Li,Aditi Paranjpe,Ping Zhou,Andrew Potter,Stacey S Huppert,Soona Shin

International journal of medical sciences 21:2770-2780 PubMed39512683

2024

LCN2 depletion aggravates sepsis-induced liver injury by regulating PTGS2-dependent ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Jiang,Zhi-Tian Jiang,Gang Zhao,Jing-Wen Cai,Jie Song,Jing Wang,Zhen Zhou,Qian Wang,Qi-Hua Ling

Open life sciences 19:20220884 PubMed39035458

2024

Isomangiferin promotes the migration and osteogenic differentiation of rat bone marrow mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bingjun Gao,Xin Cheng,Yarong Wu,Boyi Jiang

International journal of molecular sciences 25: PubMed38999957

2024

Effects of High-Mobility Group Box-1 on Mucosal Immunity and Epithelial Differentiation in Colitic Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Takamitsu Sasaki,Rina Fujiwara-Tani,Yi Luo,Ruiko Ogata,Rika Sasaki,Ayaka Ikemoto,Yukiko Nishiguchi,Chie Nakashima,Shingo Kishi,Kiyomu Fujii,Hitoshi Ohmori,Naohide Oue,Hiroki Kuniyasu

Journal of microbiology and biotechnology 34:415-424 PubMed38044675

2023

In Vitro and In Vivo Bone-Forming Effect of a Low-Molecular-Weight Collagen Peptide.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Min Hwang,Mun-Hoe Lee,Yuri Kwon,Hee-Chul Chung,Do-Un Kim,Jin-Hee Lee

Journal of controlled release : official journal of the Controlled Release Society 362:536-547 PubMed37648082

2023

Peptide delivery of a multivalent mRNA SARS-CoV-2 vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Cian M McCrudden,Lindsey Bennie,Philip Chambers,Jordan Wilson,Megan Kerr,Monika Ziminska,Hayley Douglas,Sarah Kuhn,Emma Carroll,Garrett O'Brien,Niamh Buckley,Nicholas J Dunne,Helen O McCarthy

Molecular medicine (Cambridge, Mass.) 28:52 PubMed35508992

2022

Resveratrol ameliorates liver fibrosis induced by nonpathogenic Staphylococcus in BALB/c mice through inhibiting its growth.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqin Li,Jianxia Dong,Meng Wang,Jingya Yan,Yushu Hu,Yang Liu,Yajie Pan,Hua Li
View all publications
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