Monkey IL-10 ELISA试剂盒(ab119530)
Key features and details
- Sensitivity: 1.3 U/ml
- Range: 3.9 U/ml - 250 U/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
概述
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产品名称
Monkey IL-10 ELISA试剂盒
参阅全部 IL-10 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 6 < 5% 批次间 样品 n Mean SD CV% Overall 6 < 10% -
样品类型
Cell culture supernatant, Serum -
检测类型
Sandwich (quantitative) -
灵敏度
1.3 U/ml -
范围
3.9 U/ml - 250 U/ml -
回收率
95 %
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实验步骤
Multiple steps standard assay -
种属反应性
与反应: Monkey -
产品概述
Abcam’s IL-10 Monkey ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Monkey IL-10 in cell culture supernatant and serum (baboon, cynomolgus).
This assay employs an antibody specific for Monkey IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Monkey IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Standard is recombinant protein produced in E.coli.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-monkey IL-10 monoclonal antibody 1 x 70µl IL-10 Standard lyophilized (500 U/mL upon reconstitution) 2 vials Microplate coated with monoclonal antibody to IL-10 (12 x 8 wells) 1 unit Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究领域
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功能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
组织特异性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
序列相似性
Belongs to the IL-10 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119530于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab119530.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab119530 尚未被引用在任何文献中。