Met (pY1234/pY1235) + total Met ELISA试剂盒(ab126451)
Key features and details
- Sample type: Cell Lysate, Tissue Lysate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Met (pY1234/pY1235) + total Met ELISA试剂盒
参阅全部 Met (c-Met) 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell Lysate, Tissue Lysate -
检测类型
Semi-quantitative -
检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse, Rat, Human -
产品概述
ab126451 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates. By determining phosphorylated Met protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Met (Tyr1234/1235) and total Met in human, and mouse cell lysates (help normalize the results of phospho-Met from different cell lysate being compared). A pan Met antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Met present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-Met (Tyr1234/1235) or anti-pan-Met is used to detect phosphorylated or total Met. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Met (Tyr1234/1235) or total Met bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 1000X HRP-conjugated anti-rabbit IgG 1 x 25µl 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml Detection Antibody Met 1 vial Detection Antibody Met (Y1234/1235) 1 vial Met Microplate (12 strips x 8 wells) coated with anti-pan Met antibody 1 unit Positive Control: lyophilized powder from H1993 cell lysate 1 vial Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival. -
疾病相关
Note=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
Note=Defects in MET may be associated with gastric cancer.
Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain. -
结构域
The kinase domain is involved in SPSB1 binding. -
翻译后修饰
Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. -
细胞定位
Membrane. - Information by UniProt
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别名
- AUTS9
- c met
- D249
see all -
数据库链接
- Entrez Gene: 4233 Human
- Entrez Gene: 17295 Mouse
- Entrez Gene: 24553 Rat
- Omim: 164860 Human
- SwissProt: P08581 Human
- SwissProt: P16056 Mouse
- SwissProt: P97523 Rat
- Unigene: 132966 Human
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126451于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
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Western blot - Met (pY1234/pY1235) + total Met ELISA Kit (ab126451)H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed by Western blot.
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Sandwich ELISA - Met (pY1234/pY1235) + total Met ELISA Kit (ab126451)H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126451. -
Sandwich ELISA - Met (pY1234/pY1235) + total Met ELISA Kit (ab126451)A431 cells were treated or untreated with 50 ng/ml recombinant human HGF for 5 min. Cell lysates were analyzed using ab126451. -
Sandwich ELISA - Met (pY1234/pY1235) + total Met ELISA Kit (ab126451)H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126451.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab126451 被引用在 3 文献中.
- Lee CC et al. Epithelial cell adhesion molecule (EpCAM) regulates HGFR signaling to promote colon cancer progression and metastasis. J Transl Med 21:530 (2023). PubMed: 37543570
- Useckaite Z et al. Extracellular vesicles report on the MET status of their cells of origin regardless of the method used for their isolation. Sci Rep 10:19020 (2020). PubMed: 33149187
- AbouAitah K et al. Folic acid-conjugated mesoporous silica particles as nanocarriers of natural prodrugs for cancer targeting and antioxidant action. Oncotarget 9:26466-26490 (2018). PubMed: 29899871