JNK 1/2 ELISA试剂盒(ab176646)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.1 ng/ml
- Range: 0.2 ng/ml - 20 ng/ml
- Sample type: Cell Lysate, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Mouse, Human
概述
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产品名称
JNK 1/2 ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% HEK extracts 6 2.8% 批次间 样品 n Mean SD CV% HEK extracts 3 3.3% -
样品类型
Cell Lysate, Tissue Homogenate -
检测类型
Semi-quantitative -
灵敏度
0.1 ng/ml -
范围
0.2 ng/ml - 20 ng/ml -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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产品概述
Abcam’s JNK1/2 in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of JNK1/2 protein in Human and mouse cells.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Buffer PT 1 x 15ml 50X Cell Extraction Enhancer Solution 1 x 1ml 5X Cell Extraction Buffer PTR 1 x 12ml JNK1/2 (Total) Capture Antibody 1 x 3ml JNK1/2 (Total) Detector Antibody 1 x 3ml Lyophilized JNK1/2 Control Lysate 1 vial Plate Seal 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Substrate 1 x 12ml -
研究领域
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相关性
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. -
数据库链接
- Entrez Gene: 5599 Human
- Entrez Gene: 26419 Mouse
- Entrez Gene: 116554 Rat
- SwissProt: P45983 Human
- SwissProt: Q91Y86 Mouse
- SwissProt: P49185 Rat
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176646于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
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Typical cell lysate dilution series
Example of a typical JNK1/2 cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.
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Typical recombinant protein standard curveExample of a typical JNK1/2 recombinant protein standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Linearity of dilutionLinearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of JNK1/2 are normalized and plotted.
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Comparison of JNK 1/2 expression in different cell linesCell line analysis for Total JNK1/2 from 200 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
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JNK 1/2 phosphorylation in response to anisomycin treatmentInduction of JNK1/2 (pT183/Y185) phosphorylation in HeLa cells in response to anisomycin treatment. HeLa cells were cultured in 96-well tissue culture plates and treated (30 min) with a dose-range of anisomycin before cell lysis. Data from quadruplicate measurements of JNK1/2 (pT183/Y185) are plotted and compared against Total JNK1/2 protein levels. Comparative JNK1/2 (pT183/Y185) and JNK1/2 (Total) data also shown by Western Blot.
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Sandwich ELISA - JNK 1/2 ELISA Kit (ab176646)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - JNK 1/2 ELISA Kit (ab176646)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab176646 被引用在 1 文献中.
- Costa-Rodrigues J et al. Modulation of human osteoclastogenesis and osteoblastogenesis by lycopene. J Nutr Biochem 57:26-34 (2018). PubMed: 29655028