AB264620

人XCL1 ELISA试剂盒

Human XCL1 ELISA Kit

Recombinant
SimpleStep
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Human XCL1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human XCL1 with a sensitivity of 28.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

LTN, SCYC1, XCL1, Lymphotactin, ATAC, C motif chemokine 1, Cytokine SCM-1, Lymphotaxin, SCM-1-alpha, Small-inducible cytokine C1, XC chemokine ligand 1

3 Images

Sandwich ELISA - Human XCL1 ELISA Kit (AB264620)

sELISA

Supplier Data

Sandwich ELISA - Human XCL1 ELISA Kit (AB264620)

Interpolated concentrations of native XCL1 in human PMA + Ionomycin treated PBMC cell culture supernatant samples.

Peripheral blood mononuclear cells (PBMC) were cultured in the presence (stimulated) or absence of 50 ng/mL PMA and 1 μg/mL Ionomycin for 24 hours. Cell culture supernatants were collected and assayed for the presence of XCL1. The concentrations of XCL1 were measured in duplicates, interpolated from the XCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean XCL1 concentration was determined to be 4,506 pg/mL in PMA + Ionomycin treated PBMC cell culture supernatant and not detectable in untreated samples (data not shown).

sELISA

Supplier Data

Sandwich ELISA - Human XCL1 ELISA Kit (AB264620)

Human XCL1 standard curve comparison.

Standard Curve comparison between human XCL1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human XCL1 ELISA Kit (AB264620)

Example of human XCL1 standard curve in Sample Diluent NS.

The XCL1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture media, Heparin Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 28.3 pg/mL

范围

97.48 - 2800 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human XCL1 ELISA Kit (ab264620) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of XCL1 protein in cell culture supernatant, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human XCL1 with 28.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

XCL1 (Lymphotactin) is a secreted glycosylated protein of C chemokine family. It plays a role in chemotactic activity of lymphocytes, but not monocytes or neutrophils. In the thymus, XCL1 mediates medullary accumulation of dendritic cells and contributes to T cell development which plays a role in establishment of self-tolerance. XCL2 is functionally related XCL1 homolog, these proteins share 98% amino acids sequence identity.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]

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回收率

[ { "sample": "Cell culture supernatant", "range": "101 - 109 %", "average": "= 105" }, { "sample": "Serum", "range": "96 - 100 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "89 - 92 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "85 - 88 %", "average": "= 87" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264620-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264620-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB264620-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264620-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264620-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human XCL1 Capture Antibody", "number":"AB264620-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB264620-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human XCL1 Lyophilized Recombinant Protein", "number":"AB264620-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264620-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264620-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264620-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human XCL1 Detector Antibody", "number":"AB264620-CMP09", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

XCL1 also known as lymphotactin is a chemokine with a molecular mass of approximately 10 kDa. This protein plays a role in the immune system directing the movement of cells such as T lymphocytes to sites of inflammation or infection. XCL1 is mainly expressed in activated T cells natural killer cells and some other immune cells. Its expression gets upregulated in response to certain stimuli which helps to recruit additional immune effector cells to areas where they are needed.

Biological function summary

XCL1 participates in chemotaxis guiding the migration of specific cells to enhance the immune response. Although XCL1 does not form part of a larger protein complex it interacts with its specific receptor XCR1 on target cells. This interaction triggers intracellular signaling pathways contributing to the controlled movement and positioning of immune cells in response to infection or inflammatory signals.

Pathways

XCL1 is significantly involved in the chemokine signaling pathway and plays a part in immune response regulation. This pathway shared with other chemokines like CCL2 and CCL5 orchestrates the precise movement and activity of immune cells. XCL1’s interaction with XCR1 is central to initiating downstream signaling processes which influence immune responses and inflammation.

XCL1 associates with autoimmune diseases and certain viral infections. Its dysregulation appears in conditions such as rheumatoid arthritis where the abnormal control of immune cells contributes to inflammation and tissue damage. In viral infections like HIV XCL1 helps modulate immune cell responses against the virus. XCR1 the receptor of XCL1 also plays a role in these diseases being a critical component in mediating the effects of lymphotactin in varied pathological contexts.

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产品实验方案

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靶点信息

Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment.

See full target information XCL1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 10:1189134 PubMed37359017

2023

A novel predictive model for the recurrence of pediatric alopecia areata by bioinformatics analysis and a single-center prospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanquan Zheng,Yingli Nie,Jingjing Lu,Hong Yi,Guili Fu

View all publications

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websiteProtocolBooklet

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人XCL1 ELISA试剂盒

Human XCL1 ELISA Kit

Sandwich ELISA - Human XCL1 ELISA Kit (AB264620)

Sandwich ELISA - Human XCL1 ELISA Kit (AB264620)

Sandwich ELISA - Human XCL1 ELISA Kit (AB264620)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台