AB168548

人Von Willebrand Factor ELISA试剂盒(with plasma controls)

Name: 人Von Willebrand Factor ELISA试剂盒 (with plasma controls) (ab168548)| Abcam中文官网
Brand: Abcam Limited
SKU: AB168548
Price: 7929 CNY
Availability: InStock

Human Von Willebrand Factor ELISA Kit (with plasma controls)

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(3 Publications)

Human Von Willebrand Factor ELISA Kit (with plasma controls) is a Sandwich (quantitative) ELISA for the measurement of Human Von Willebrand Factor (with plasma controls) in Human in Biofluids samples.

查看别名

F8VWF, VWF, von Willebrand factor, vWF

1 Images

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (with plasma controls) (AB168548)

sELISA

Supplier Data

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (with plasma controls) (AB168548)

Representative Standard Curve using ab168548

关键信息

检测方法

Colorimetric

样品类型

Plasma, Serum

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

> 0.09 mIU/mL

范围

0.625 - 40 mIU/mL

检测时间

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Abcam's Von Willebrand Factor (VWF) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Von Willebrand Factor levels in cell culture supernatants, plasma and serum.

A Von Willebrand Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Von Willebrand Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Von Willebrand Factor captured in plate.

Get higher sensitivity in only 90 minutes with Human Von Willebrand Factor ELISA Kit (ab189571) from our SimpleStep ELISA^® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab168548-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB168548-CMP03", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB168548-CMP02", "productcode":"" }, { "size":"1 x 60 µL", "name":"100X Biotinylated Human Von Williebrand Factor Antibody", "number":"AB168548-CMP10", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB168548-CMP05", "productcode":"" }, { "size":"1 x 96 Test", "name":"Von Williebrand Factor Microplate (12 x 8 well strips)", "number":"AB168548-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Low control (Depleted Human Plasma)", "number":"AB168548-CMP11", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB168548-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB168548-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive control (Reference Plasma Control)", "number":"AB168548-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Von Williebrand Factor Standard", "number":"AB168548-CMP09", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB168548-CMP07", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

Please refer to protocols

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

The protein expressed by the gene VWF is important for maintaining hemostasis. It promotes platelet adhesion at sites of vascular injury by forming a molecular bridge between the sub-endothelial collagen matrix and the platelet-surface receptor complex GPIb-IX-V. Additionally, it acts as a chaperone for coagulation factor VIII, delivering it to the injury site, stabilizing its heterodimeric structure, and protecting it from premature clearance from plasma. This supplementary information is collated from multiple sources and compiled automatically.

See full target information VWF

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 8:817305 PubMed35087853

2022

The Impact of Age and BMI on the VWF/ADAMTS13 Axis and Simultaneous Thrombin and Plasmin Generation in Hospitalized COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Kiruphagaran Thangaraju,Upendra Katneni,Imo J Akpan,Kenichi Tanaka,Tiffany Thomas,Saini Setua,Julie A Reisz,Francesca Cendali,Fabia Gamboni,Travis Nemkov,Stacie Kahn,Alexander Z Wei,Jacob E Valk,Krystalyn E Hudson,David J Roh,Chiara Moriconi,James C Zimring,Angelo D'Alessandro,Steven L Spitalnik,Richard O Francis,Paul W Buehler

Cells 10: PubMed34571942

2021

Biological and Clinical Factors Contributing to the Metabolic Heterogeneity of Hospitalized Patients with and without COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Angelo D'Alessandro,Tiffany Thomas,Imo J Akpan,Julie A Reisz,Francesca I Cendali,Fabia Gamboni,Travis Nemkov,Kiruphagaran Thangaraju,Upendra Katneni,Kenichi Tanaka,Stacie Kahn,Alexander Z Wei,Jacob E Valk,Krystalyn E Hudson,David Roh,Chiara Moriconi,James C Zimring,Eldad A Hod,Steven L Spitalnik,Paul W Buehler,Richard O Francis

Research square : PubMed34013258

2021

Biological and Clinical Factors contributing to the Metabolic Heterogeneity of Hospitalized Patients with and without COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Angelo D'Alessandro,Imo Akpan,Tiffany Thomas,Julie Reisz,Francesca Cendali,Fabia Gamboni,Travis Nemkov,Kiruphagaran Thangaraju,Upendra Katneni,Kenichi Tanaka,Stacie Kahn,Alexander Wei,Jacob Valk,Krystalyn Hudson,David Roh,Chiara Moriconi,James Zimring,Eldad Hod,Steven Spitalnik,Paul Buehler,Richard Francis

View all publications

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人Von Willebrand Factor ELISA试剂盒(with plasma controls)

Human Von Willebrand Factor ELISA Kit (with plasma controls)

Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (with plasma controls) (AB168548)