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AB234577

人Vitronectin/S-Protein ELISA试剂盒

Human Vitronectin/S-Protein ELISA Kit

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(1 Publication)

Human Vitronectin/S-Protein ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Vitronectin/S-Protein with a sensitivity of 19.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Vitronectin, VN, S-protein, Serum-spreading factor, V75, VTN

3 Images
Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)

Representative image of Human Vitronectin/S-Protein ELISA Kit (ab234577)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Vitronectin/S-Protein ELISA Kit Capture Antibody

- 10X Human Vitronectin/S-Protein Detector Antibody

- 10X Human Vitronectin/S-Protein ELISA Kit Lyophilised Purified Protein (2 vials)

- Human Vitronectin/S-Protein ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)

Example of human Vitronectin standard curve in 1X Cell Extraction Buffer PTR.

The Vitronectin standard curve was prepared as described in Section 10.

Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vitronectin/S-Protein ELISA Kit (AB234577)

Interpolated concentrations of native Vitronectin in human serum samples.

The concentrations of Vitronectin were measured in duplicates, interpolated from the Vitronectin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitronectin concentration was determined to be 234.2 μg/mL in serum.

关键信息

检测方法

Colorimetric

样品类型

Serum

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 19.9 pg/mL

范围

0.06 - 4 ng/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

Human Vitronectin/S-Protein ELISA Kit (ab234577) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Vitronectin/S-Protein protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vitronectin/S-Protein with 19.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

精确度

[ { "reproducibilityType": "Intra", "sample": "Recombinant Protein", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]

回收率

[ { "sample": "Serum", "range": "107 - 119.2 %", "average": "= 114.9" } ]

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234577-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234577-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234577-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB234577-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234577-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234577-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234577-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB234577-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Vitronectin/S-Protein Capture Antibody", "number":"AB234577-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Vitronectin/S-Protein Lyophilized Recombinant Protein", "number":"AB234577-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB234577-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234577-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Vitronectin/S-Protein Detector Antibody", "number":"AB234577-CMP03", "productcode":"" } ] } } }

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C

产品实验方案

靶点信息

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
See full target information Vitronectin

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 13:3897-3913 PubMed37441594

2023

Macrophage promotes fibroblast activation and kidney fibrosis by assembling a vitronectin-enriched microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Yiling Peng,Li Li,Jingyue Shang,Haili Zhu,Jinlin Liao,Xue Hong,Fan Fan Hou,Haiyan Fu,Youhua Liu
View all publications
websiteProtocolBooklet
en

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