人VCAM-1 ELISA试剂盒(without plates) (ab47355)
概述
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产品名称
人VCAM-1 ELISA试剂盒(without plates) -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.6 ng/ml -
范围
1.56 ng/ml - 50 ng/ml -
检测时间
1h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s VCAM-1 Human in vitro ELISA Set is designed for the quantitative measurement of VCAM-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure human VCAM-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The VCAM-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human VCAM1
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 10 x 96 tests 15 x 96 tests 5 x 96 tests Chromogen TMB Substrate Solution 4 vials 6 vials 2 vials Detection biotinylated anti-VCAM-1 antibody 2 x 0.55ml 3 x 0.55ml 1 x 0.55ml Streptavidin-HRP 2 vials 3 vials 1 vial VCAM-1 Capture Antibody 2 x 0.5ml 3 x 0.5ml 1 x 0.5ml VCAM1 standard 10 vials 15 vials 5 vials -
研究领域
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相关性
VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells. -
细胞定位
Cell Membrane; single-pass type I membrane protein -
别名
- CD106
- CD106 antigen
- INCAM 100
see all -
数据库链接
- Entrez Gene: 7412 Human
- SwissProt: P19320 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47355于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab47355 被引用在 4 文献中.
- Lázaro APP et al. Predictors of mortality in critically ill patients with COVID-19 and diabetes. Braz J Med Biol Res 56:e12728 (2023). PubMed: 37585916
- Meneses GC et al. Endothelial biomarkers as predictors for haemodialysis need in severe leptospirosis patients (Weil's disease). Trop Med Int Health 27:727-734 (2022). PubMed: 35761507
- Tessarolo LD et al. Endothelial activation is associated with albuminuria in multibacillary leprosy. Rev Inst Med Trop Sao Paulo 63:e44 (2021). PubMed: 34161550
- Lino DOC et al. Interleukin-6 and adhesion molecules VCAM-1 and ICAM-1 as biomarkers of post-acute myocardial infarction heart failure. Braz J Med Biol Res 52:e8658 (2019). PubMed: 31778438