人uPA Receptor/U-PAR ELISA试剂盒(URKR) (ab119612)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人uPA Receptor/U-PAR ELISA试剂盒(URKR)
参阅全部 uPA Receptor/U-PAR 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
< 4 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human uPA Receptor/U-PAR (URKR) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human uPA Receptor/U-PAR (URKR) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
A uPA Receptor/U-PAR (URKR) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for uPA Receptor/U-PAR(URKR) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human uPA Receptor amount of sample captured in plate.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human uPA Receptor antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human uPA Receptor antibody 1 x 100µl Lyophilized recombinant Human uPA Receptor standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
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功能
Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. -
组织特异性
Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain. -
序列相似性
Contains 3 UPAR/Ly6 domains. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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别名
- CD 87
- CD87
- CD87 antigen
see all -
数据库链接
- Entrez Gene: 5329 Human
- Omim: 173391 Human
- SwissProt: Q03405 Human
- Unigene: 466871 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119612于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab119612 被引用在 2 文献中.
- Nantajit D et al. Prognostic significance of uPA and uPAR expression in patients with cervical cancer undergoing radiotherapy. Oncol Lett 21:423 (2021). PubMed: 33850564
- Hamie L et al. SuPAR, a potential inflammatory mediator in psoriasis pathogenesis. Clin Exp Pharmacol Physiol 47:1705-1712 (2020). PubMed: 32558953