AB226904

人U-Plasminogen活化剂ELISA试剂盒

Human U-Plasminogen Activator ELISA Kit

Recombinant
SimpleStep
了解详情

Be the first to review this product! Submit a review

(1 Publication)

Human U-Plasminogen Activator ELISA Kit is a single-wash 90-min Simplestep used to quantify Human U-Plasminogen Activator with a sensitivity of 4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Urokinase-type plasminogen activator, U-plasminogen activator, uPA, PLAU

7 Images

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

sELISA

Supplier Data

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Interpolated concentrations of native U-Plasminogen Activator in Human HEPG2 and PC-3 cell culture supernatant and urine samples.

The concentrations of U-Plasminogen Activator were measured in duplicates, interpolated from the U-Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 supernatant 25%, PC-3 supernatant 1 : 250, and Urine 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 10 ng/mL in HepG2 supernatant, 865 ng/mL in PC-3 supernatant, and 73 ng/mL in Urine.

sELISA

Supplier Data

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Interpolated concentrations of native U-Plasminogen Activator in Human serum and plasma samples.

The concentrations of U-Plasminogen Activator were measured in duplicates, interpolated from the U-Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (heparin) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 439 pg/mL in serum, 732 pg/mL in plasma (citrate), 678 pg/mL in plasma (heparin), and 871 pg/ml in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Interpolated concentrations of spiked U-Plasminogen Activator in Human serum and plasma samples.

sELISA

Supplier Data

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Serum from ten individual healthy Human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 500 pg/mL with a range of 265 – 745 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Example of Human U-Plasminogen Activator standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Interpolated concentrations of native U-Plasminogen Activator in Human PC-3 cell extract based on a 31.3 μg/mL extract load.

The concentrations of U-Plasminogen Activator were measured in duplicate and interpolated from the U-Plasminogen Activator standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 4689 pg/mL in PC-3 Cell Extract.

sELISA

Supplier Data

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Example of Human U-Plasminogen Activator standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Urine, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 4 pg/mL

范围

62.5 - 4000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

产品详情

Human U-Plasminogen Activator ELISA Kit (ab226904) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of U-Plasminogen Activator protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human U-Plasminogen Activator with 4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" } ]

style

left-column

回收率

[ { "sample": "Cell culture supernatant", "range": "96 - 113 %", "average": "= 108" }, { "sample": "Serum", "range": "85 - 90 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "88 - 93 %", "average": "= 91" }, { "sample": "Urine", "range": "97 - 115 %", "average": "= 106" }, { "sample": "Cell culture extracts", "range": "83 - 86 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "86 - 88 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "89 - 90 %", "average": "= 89" } ]

style

left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab226904-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB226904-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB226904-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB226904-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB226904-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB226904-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB226904-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human U-Plasminogen Activator Lyophilized Recombinant Protein", "number":"AB226904-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human U-Plasminogen Activator Capture Antibody", "number":"AB226904-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB226904-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human U-Plasminogen Activator Detector Antibody", "number":"AB226904-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB226904-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB226904-CMP06", "productcode":"" } ] } } }

style

left-column

性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Urokinase also known as urokinase-type plasminogen activator (uPA) is a serine protease with a molecular mass of approximately 54 kDa. It is expressed in various tissues including the kidneys liver and blood vessels. Urokinase initially forms as pro-urokinase an inactive precursor that can convert into the active enzyme. Researchers often use assays like the direct assay to measure urokinase activity due to its role in breaking down fibrin and modulating cell adhesion and migration.

Biological function summary

Something particularly interesting about urokinase is its role in converting plasminogen to plasmin a critical enzyme in the process of fibrinolysis. Moreover it is part of a larger enzymatic complex involving the urokinase receptor (uPAR) which localizes plasminogen activation to cell surfaces. This complex mediates extracellular matrix degradation aiding in important cellular processes like tissue remodeling and wound healing.

Pathways

Urokinase plays a critical role in the fibrinolytic pathway which involves the breakdown of blood clots. It also interacts with the extracellular matrix pathway by activating plasminogen which can degrade matrix components. In these pathways urokinase closely interacts with proteins such as plasmin and its inhibitor plasminogen activator inhibitor-1 (PAI-1) which regulate the activity and localization of proteolytic events.

Urokinase is strongly linked to conditions such as deep vein thrombosis and cancer metastasis. Its overexpression or dysregulation often leads to excessive tissue degradation influencing metastasis progression. Urokinase is connected to the urokinase receptor (uPAR) in cancer facilitating cancer cell invasion and migration. Understanding its behavior in these contexts is critical for developing therapeutic strategies and targeting treatments against these diseases.

style

left-column

产品实验方案

style

left-column

靶点信息

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

See full target information Urokinase-type plasminogen activator

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35269955

2022

Ficolin-2 Plasma Level Assesses Liver Fibrosis in Non-Alcoholic Fatty Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo J Giraudi,Noel Salvoza,Deborah Bonazza,Carlo Saitta,Daniele Lombardo,Biagio Casagranda,Nicolò de Manzini,Teresa Pollicino,Giovanni Raimondo,Claudio Tiribelli,Silvia Palmisano,Natalia Rosso

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

人U-Plasminogen活化剂ELISA试剂盒

Human U-Plasminogen Activator ELISA Kit

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (AB226904)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台