人TSC-1 ELISA试剂盒(ab223594)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.54 pg/ml
- Range: 4.69 pg/ml - 300 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人TSC-1 ELISA试剂盒
参阅全部 TSC-1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Supernatant 8 4.8% 批次间 样品 n Mean SD CV% Supernatant 3 8.7% -
样品类型
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1.54 pg/ml -
范围
4.69 pg/ml - 300 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 101 95% - 108% Serum 102 90% - 118% EDTA Plasma 101 85% - 109% Cit plasma 102 83% - 117% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human TSC-1 ELISA Kit (ab223594) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TSC-1 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TSC-1 with 1.54 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
说明
TSC-1 (Eotaxin 3 / CCL26) is a small cytokine within the CC chemokine family coded by a gene located on human chromosome 7. TSC-1 is also know by Macrophage inflammatory protein 4-alpha, Thymic stroma chemokine-1 and IMAC. It has known expression in heart, lung and ovarian tissue. TSC-1 expression can be induced by IL-4 stimulation which then can activate chemotaxic activity of eosinophils and basophiles by binding to the cell surface chemokine receptor CCR3.
-
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml 10X Human TSC-1 Detector Antibody 1 x 600µl 10X Human TSC-1 Capture Antibody 1 x 600µl Human TSC-1 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
-
功能
Chemotactic for eosinophils and basophils. Binds to CCR3. -
组织特异性
Ubiquitously expressed at low levels in various tissues including heart and ovary. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
细胞定位
Secreted. - Information by UniProt
-
别名
- C-C motif chemokine 26
- CC chemokine IMAC
- CCL 26
see all -
数据库链接
- Entrez Gene: 10344 Human
- Omim: 604697 Human
- SwissProt: Q9Y258 Human
- Unigene: 131342 Human
相关产品
-
Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223594于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
-
Other - Human TSC-1 ELISA Kit (ab223594)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of Human Eotaxin 3 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Eotaxin 3 in human A549 cell culture supernatant samples.The concentrations of Eotaxin 3 were measured in duplicates, interpolated from the Eotaxin 3 standard curves and corrected for sample dilution. Undiluted human A549 supernatant: 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin 3 concentration was determined to be 5559.17 pg/mL.
-
Interpolated concentrations of spiked Eotaxin 3 in serum and plasma.Interpolated concentrations of spiked Eotaxin 3 in human serum 50%, plasma (citrate) 50% and plasma (EDTA) 50%. The concentrations of Eotaxin 3 were measured in duplicate and interpolated from the Eotaxin 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin 3 concentration was determined to be 161 pg/mL in human serum and 320.02 pg/mL in citrate plasma, 318.05 pg/ml in EDTA plasma.
-
Sandwich ELISA - Human TSC-1 ELISA Kit (ab223594)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab223594 尚未被引用在任何文献中。