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AB323931

Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit

Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit

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Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit is a Sandwich ELISA for the measurement of Human Tryptase alpha/beta-1 (TPSAB1) in Human in Biofluids, Cell Culture Media samples.

查看别名

TPS1, TPS2, TPSB1, TPSAB1, Tryptase alpha/beta-1, Tryptase-1, Tryptase I, Tryptase alpha-1

6 Images
Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)

Interpolated concentration of native Tryptase alpha/beta-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)

Interpolated concentration of native Tryptase alpha/beta-1 was measured in duplicate at different sample concentrations. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)

Example of human Tryptase alpha/beta-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)

Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Tryptase alpha/beta-1 concentration was determined to be 1,994 pg/mL with a range of 553 – 3,713 pg/mL.

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)

Example of human Tryptase alpha/beta-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (AB323931)

Interpolated concentration of native Tryptase alpha/beta-1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% stimulated PBMC supernatant (1.5% PHA-M for 46 hrs) and unstimulated PBMC supernatant (mock treated). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

关键信息

检测方法

Colorimetric

样品类型

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

反应种属

Human

检测类型

Sandwich

灵敏度

= 3.2 pg/mL

范围

12.5 - 800 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Tryptase alpha/beta-1 (TPSAB1) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tryptase alpha/beta-1 (TPSAB1) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Tryptase alpha/beta-1 (TPSAB1) with 3.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Singlewash protocol reduces assay time to 90 minutes or less.

High sensitivity, specificity and reproducibility from superior antibodies.

Fully validated in biological samples.

96wells plate breakable into 12 x 8 wells strips, available in 20pack (10 x 96well plates).

Also available in a fully validated 384well format.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.6" } ]
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回收率

[ { "sample": "Serum", "range": "98 - 113 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "97 - 103 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "113 - 124 %", "average": "= 118" }, { "sample": "Heparin Plasma", "range": "91 - 108 %", "average": "= 97" }, { "sample": "Cell culture supernatant", "range": "98 - 100 %", "average": "= 99" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed : 18854315).
See full target information TPSAB1
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websiteProtocolBooklet
en
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