AB322439

Human TROP2 ELISA Kit- Extracellular

Recombinant
SimpleStep
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Human TROP2 ELISA Kit- Extracellular is a single-wash 90-min SimpleStep ELISA used to quantify Human TROP2 with a sensitivity of 1.623 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab326013

GA733-1, M1S1, TROP2, TACSTD2, Tumor-associated calcium signal transducer 2, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1

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Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

sELISA

Supplier Data

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TROP2 concentration was determined to be 116.15 pg/mL with a range of 88.9 – 140.6 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

Interpolated concentration of native TROP2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 6.25% A-431 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

Interpolated concentration of native TROP2 was measured in duplicate at different sample concentrations. Undiluted samples are 12.5% A-431 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

Example of human TROP2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human TROP2 ELISA Kit- Extracellular (AB322439)

Example of human TROP2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

反应种属

Human

检测类型

Sandwich

Quantitative

灵敏度

= 1.623 pg/mL

范围

9.375 - 600 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human TROP2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TROP2 protein in Serum, Cit Plasma, EDTA Plasma, and Cell culture supernatant. Quantitate Human TROP2 with 1.623 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

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回收率

[ { "sample": "Serum", "range": "86 - 97 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "104 - 109 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "86 - 113 %", "average": "= 100" }, { "sample": "Cell culture supernatant", "range": "88 - 93 %", "average": "= 91" } ]

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规格

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab322439-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB322439-CMP06" }, { "size":"20 x 1 Vial", "name":"Human TROP2 Lyophilized Recombinant Protein", "number":"AB322439-CMP10" }, { "size":"10 x 1 Vial", "name":"Lyophilized Human TROP2 Capture Antibody", "number":"AB322439-CMP01" }, { "size":"10 x 600 µL", "name":"10X Human TROP2 Detector Antibody", "number":"AB322439-CMP09" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB322439-CMP04" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB322439-CMP03" }, { "size":"2 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB322439-CMP08" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322439-CMP11" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322439-CMP02" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322439-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322439-CMP12" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab322439-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322439-CMP06" }, { "size":"1 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB322439-CMP08" }, { "size":"2 x 1 Vial", "name":"Human TROP2 Lyophilized Recombinant Protein", "number":"AB322439-CMP10" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human TROP2 Capture Antibody", "number":"AB322439-CMP01" }, { "size":"1 x 600 µL", "name":"10X Human TROP2 Detector Antibody", "number":"AB322439-CMP09" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB322439-CMP04" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB322439-CMP03" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322439-CMP12" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322439-CMP02" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322439-CMP11" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB322439-CMP07" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab322439-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322439-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TROP2 Lyophilized Recombinant Protein", "number":"AB322439-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human TROP2 Capture Antibody", "number":"AB322439-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TROP2 Detector Antibody", "number":"AB322439-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322439-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322439-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"Cell Extraction Buffer SSW", "number":"AB322439-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322439-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322439-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB322439-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB322439-CMP03", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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产品实验方案

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靶点信息

May function as a growth factor receptor.

See full target information TACSTD2

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websiteProtocolBooklet

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Human TROP2 ELISA Kit- Extracellular