人Transferrin ELISA试剂盒(ab108911)
Key features and details
- Sensitivity: 89 ng/ml
- Range: 0.4 µg/ml - 6.25 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
-
产品名称
人Transferrin ELISA试剂盒
参阅全部 Transferrin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4.2% 批次间 样品 n Mean SD CV% Overall 10.1% -
样品类型
Serum, Plasma -
检测类型
Competitive -
灵敏度
= 89 ng/ml -
范围
0.4 µg/ml - 6.25 µg/ml -
回收率
96 %
-
检测时间
2h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Transferrin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in plasma and serum samples.
A Transferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Transferrin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Transferrin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 6X Biotinylated Human Transferrin (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Human Transferrin Standard (Lyophilized) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml Transferrin Microplate (12 x 8 well strips) 1 unit -
研究领域
-
功能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
组织特异性
Expressed by the liver and secreted in plasma. -
疾病相关
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
序列相似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
细胞定位
Secreted. - Information by UniProt
-
别名
- Apotransferrin
- Beta 1 metal binding globulin
- Beta-1 metal-binding globulin
see all -
数据库链接
- Entrez Gene: 7018 Human
- Omim: 190000 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108911于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
-
Typical Standard Curve
Representative Standard Curve using ab108911
数据表及文件
-
SDS download
-
Datasheet download
文献 (15)
ab108911 被引用在 15 文献中.
- Oldfield L et al. Blood levels of adiponectin and IL-1Ra distinguish type 3c from type 2 diabetes: Implications for earlier pancreatic cancer detection in new-onset diabetes. EBioMedicine 75:103802 (2022). PubMed: 34990893
- Hu Y et al. Clinical Characteristics, Iron Metabolism and Neuroinflammation: New Insight into Excessive Daytime Sleepiness in Parkinson's Disease. Neuropsychiatr Dis Treat 17:2041-2051 (2021). PubMed: 34188474
- Rodríguez-Mortera R et al. Higher Hepcidin Levels in Adolescents with Obesity Are Associated with Metabolic Syndrome Dyslipidemia and Visceral Fat. Antioxidants (Basel) 10:N/A (2021). PubMed: 34065056
- Mateos J et al. Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection. Sci Rep 10:3844 (2020). PubMed: 32123229
- Lian TH et al. Tremor-Dominant in Parkinson Disease: The Relevance to Iron Metabolism and Inflammation. Front Neurosci 13:255 (2019). PubMed: 30971879