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人Transferrin ELISA试剂盒(ab108902)

Reviews (1)Q&A (2)References (4)
Typical Standard Curve

    Key features and details

    • Sensitivity: 0.78 ng/ml
    • Range: 6.25 ng/ml - 50 ng/ml
    • Sample type: Milk, Saliva, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    概述

    • 产品名称

      人Transferrin ELISA试剂盒
      参阅全部 Transferrin 试剂盒

    • 检测方法

      Colorimetric

    • 精确度

      批次内
      样品 n Mean SD CV%
      Overall 4.5%
      批次间
      样品 n Mean SD CV%
      Overall 9.6%

    • 样品类型

      Saliva, Milk, Urine

    • 检测类型

      Sandwich (quantitative)

    • 灵敏度

      = 0.78 ng/ml

    • 范围

      6.25 ng/ml - 50 ng/ml

    • 回收率

      97 %

    • 检测时间

      4h 00m

    • 实验步骤

      Multiple steps standard assay

    • 种属反应性

      与反应: Human

    • 产品概述

      Abcam’s Tranferrin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in milk, urine, saliva, CSF, cell culture, cell lysate, and tissue samples.


      A Tranferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tranferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tranferrin captured in plate.


      Get results in 90 minutes with Human Transferrin ELISA Kit (ab187391) from our SimpleStep ELISA® range.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • 经测试应用

      适用于: Sandwich ELISAmore details

    • 平台

      Microplate

    性能

    相关产品

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab108902于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Sandwich ELISA
    Use at an assay dependent concentration.
    说明
    Sandwich ELISA
    Use at an assay dependent concentration.

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108902.

    文献 (4)

    发表研究结果有使用 ab108902？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab108902 被引用在 4 文献中.

    • Kaur H  et al. Higher CSF Ferritin Heavy-Chain (Fth1) and Transferrin Predict Better Neurocognitive Performance in People with HIV. Mol Neurobiol 58:4842-4855 (2021). PubMed: 34195939
    • Hazlett HF  et al. Altered iron metabolism in cystic fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas aeruginosa survival. Sci Rep 10:10935 (2020). PubMed: 32616918
    • Mehta SR  et al. Cerebrospinal fluid cell-free mitochondrial DNA is associated with HIV replication, iron transport, and mild HIV-associated neurocognitive impairment. J Neuroinflammation 14:72 (2017). ELISA ; Human . PubMed: 28359324
    • Patton SM  et al. Cerebrospinal fluid (CSF) biomarkers of iron status are associated with CSF viral load, antiretroviral therapy, and demographic factors in HIV-infected adults. Fluids Barriers CNS 14:11 (2017). ELISA ; Human . PubMed: 28427421

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Abreview for Transferrin Human ELISA Kit

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    abreview image

    Sample: Human Cell (HepG2)
    Specification: HepG2
    Amount of Sample: 0.1ug standard µg
    Type of Kit Used: ELISA
    Positive control: Provided transferrin standard
    Did you use the Abcam Kit protocol?: Yes

    Additional data
    Additional Notes: Recognized human transferrin. Did not recognize bovine transferrin in FBS.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 04 2012

    Question

    I am using transferrin ligands conjugated to Alexa, will this interfere in the kit?

    Read More
    Answer

    Thank you for your patience in awaiting our reply in the matter of compaibility with the kit. It is difficulty to know whether this will work. If the conjugation block the antibody binding site, it will interfere the assay. If not, it will be fine. Since it is a polyclonal antibody in the kit, the epitope has not been mapped so it would have to be tried to determine this.

    Please let me know if I can be of further assistance.

    Read More

    Question

    Dear Sir/madam,

    I am planning to buy 3 * 3 kits i.e 9 kits in total for detecting 3 different things as following:


    Albumin Human ELISA Kit

    Transferrin Human ELISA Kit

    beta 2 Microglobulin Human ELISA Kit

    I have some questions before I do this step and your help is highly appreciated. My questions are:


    1. I do measure above targets in peritoneal dialysate (PD) fluid and plasma. There are 2 types of ELISA in your company (Cell culture supernatant, Saliva, Milk, Urine Elisa which is more sensitive than plasma, serum Elisa). I am thinking to buyCell culture supernatant, Saliva, Milk, Urine Elisa, then I use them for measuring in Pd fluid and plasma . Is it applicable to use cell culturesupernatant Elisa for both?

    2. I am student and I am planing to buy the rest 6 Elisa after I get good results from 1st 3 Elisa that I use like test. Do I get good discount?

    3. I found sELISA, cELISA,...etc. what these small s and creferto?

    4. Is the only difference betweenab108885 andab99977, is difference in sensitivity?

    Note: I have goodexperience with abcam. I bought MMP-13 Elisa kit from abcam few months ago and it worked perfectly. When I did biocompare search, then I found tempting prices in some companies, but Abcam was my first decision to contact after this good past experience.

    Thanks,
    Samir

    Read More
    Answer

    Thank you for your inquiry and you positive feedback.

    Please see my answers to your questions below:

    1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially optimised for cell culture samples and I recommend to choose them for the experiment.

    2. We do offer bulk oder discounts. If you order 5 or more products you will get a discount. For 6 products you will currently get a 5% discount. Please do not forget to mention that when ordering. If you are planning to order more than 10 products, please contact our customer service department with the numbers and they might be able to offer you a higher discount.

    3. I can confirm that c stands for competitive ELISA and s stands for sandwich ELISA. In a competitive ELISA the competitor is labeled with biotin and the amount of the bound competitor is measured. This means that a high OD indicates a low amount of the protein of interest in your sample. A low OD means that the competitor has been ousted and there is a high amount of the protein of interest in the sample.

    4. I can confirm that the main difference between these two kits is the sensitivity. Both are suitable for cell culture supernatant, urine, serum, plasma.

    Our Abpromise guarantee applies to all these kits.

    https://www.abcam.com/abpromise

    I hope this information is helpful and wish you good luck with your experiments. Please do not hesitate to contact me again with any further questions.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com