人TRAIL ELISA试剂盒(CD253) (ab46074)
Key features and details
- Sensitivity: 64 pg/ml
- Range: 93.75 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人TRAIL ELISA试剂盒(CD253)
参阅全部 TRAIL 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 40 1500pg/ml 82.9 5.5% B 33 116.3pg/ml 8.7 7.4% 批次间 样品 n Mean SD CV% A 12 1619pg/ml 62.8 3.8% B 12 162.8pg/ml 13.1 8% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 64 pg/ml -
范围
93.75 pg/ml - 3000 pg/ml -
检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human TRAIL (CD253) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab46074) is designed for the quantitative measurement of TRAIL in cell culture supernatants, buffered solutions or Human serum, plasma, and other body fluids.
A monoclonal antibody specific for TRAIL has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TRAIL concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TRAIL are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TRAIL present in the samples.
This kit will recognize both endogenous and recombinant Human TRAIL.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Biotinylated anti-TRAIL Red 2 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml Control Silver 4 vials 2 vials HRP Diluent Red 1 x 23ml 1 x 12ml Plastic Plate Covers 4 units 2 units Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl TRAIL Microplate (12 x 8 well strips) 2 units 1 unit TRAIL Standard (Lyophilized) Yellow 4 vials 2 vials -
研究领域
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相关性
TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TRAIL may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. -
细胞定位
Cell Membrane; single-pass type II membrane protein. -
别名
- Apo 2 ligand
- APO2L
- CD253
see all -
数据库链接
- Entrez Gene: 8743 Human
- SwissProt: P50591 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46074于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
图片
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TRAIL measured in undiluted biological fluids (duplicates +/- SD).
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Representative Standard Curve using ab46074
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab46074 被引用在 6 文献中.
- King JL et al. Injectable pH Thermo-Responsive Hydrogel Scaffold for Tumoricidal Neural Stem Cell Therapy for Glioblastoma Multiforme. Pharmaceutics 14:N/A (2022). PubMed: 36297678
- Kitamura Y et al. Anti-EGFR VHH-armed death receptor ligand-engineered allogeneic stem cells have therapeutic efficacy in diverse brain metastatic breast cancers. Sci Adv 7:N/A (2021). PubMed: 33658202
- Read SA et al. Macrophage Coordination of the Interferon Lambda Immune Response. Front Immunol 10:2674 (2019). PubMed: 31798594
- Kurt IC et al. KDM2B, an H3K36-specific demethylase, regulates apoptotic response of GBM cells to TRAIL. Cell Death Dis 8:e2897 (2017). PubMed: 28661478
- Wang J et al. Silencing the epigenetic silencer KDM4A for TRAIL and DR5 simultaneous induction and antitumor therapy. Cell Death Differ 23:1886-1896 (2016). Human . PubMed: 27612013
- Greenwood EJ et al. Simian Immunodeficiency Virus Infection of Chimpanzees (Pan troglodytes) Shares Features of Both Pathogenic and Non-pathogenic Lentiviral Infections. PLoS Pathog 11:e1005146 (2015). Sandwich ELISA . PubMed: 26360709