JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB113323

人TNFSF18 / GITRL ELISA试剂盒

Human TNFSF18 / GITRL ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human TNFSF18 / GITRL ELISA Kit is a Sandwich ELISA for the measurement of Human TNFSF18 / GITRL in Human in Biofluids, Cell Culture Media samples.

查看别名

AITRL, GITRL, TL6, UNQ149/PRO175, TNFSF18, Tumor necrosis factor ligand superfamily member 18, Activation-inducible TNF-related ligand, Glucocorticoid-induced TNF-related ligand, hGITRL

1 Images
Sandwich ELISA - Human TNFSF18 / GITRL ELISA Kit (AB113323)
  • sELISA

Unknown

Sandwich ELISA - Human TNFSF18 / GITRL ELISA Kit (AB113323)

Representative standard curve using ab113323.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

灵敏度

< 25 pg/mL

范围

41 - 10000 pg/mL

检测平台

Microplate

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Human TNFSF18 / GITRL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNFSF18 / GITRL in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for human TNFSF18 / GITRL coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFSF18 / GITRL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFSF18 / GITRL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFSF18 / GITRL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

style
left-column

回收率

[ { "sample": "Cell culture supernatant", "range": "67 - 100 %", "average": "= 87.83" }, { "sample": "Serum", "range": "108 - 123 %", "average": "= 116.9" }, { "sample": "Plasma", "range": "67 - 100 %", "average": "= 87.69" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113323-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113323-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113323-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human GITR Ligand Standard (lyophilized)", "number":"AB113323-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB113323-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113323-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB113323-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human GITR Ligand", "number":"AB113323-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113323-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TNFSF18 Ligand Microplate (12 x 8 wells)", "number":"AB113323-CMP07", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
style
left-column
style
left-column

产品实验方案

style
left-column

靶点信息

Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T-cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1 (PubMed : 23892569). Promotes leukocyte adhesion to endothelial cells (PubMed : 23892569). Regulates migration of monocytes from the splenic reservoir to sites of inflammation (By similarity).
See full target information TNFSF18
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

搭配使用产品

Proteins & Peptides

AB271791

Recombinant Human VSIG8 protein (Fc tag C-Terminus + Avi tag C-Terminus)

SDS-PAGE - Recombinant Human VSIG8 protein (Fc tag C-Terminus + Avi tag C-Terminus) (AB271791)

  • 0
  • 0
Primary Antibodies

AB279982

Anti-TNFSF18/GITRL antibody [EPR23451-201] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-TNFSF18/GITRL antibody [EPR23451-201] - BSA and Azide free (Capture) (AB279982)

  • 0
  • 0
Proteins & Peptides

AB307487

Recombinant Human TNFAIP3 Protein

Mass Spectrometry - Recombinant Human TNFAIP3 Protein (AB307487)

  • 0
  • 0
Proteins & Peptides

AB319985

Recombinant Mouse Prealbumin Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse Prealbumin Protein Standard (His tag) (AB319985)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com