JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181421

人TNF alpha ELISA试剂盒

Human TNF alpha ELISA Kit

5

(4 Reviews)

|

(319 Publications)

Human TNF alpha ELISA kit is a single-wash 90-min SimpleStep ELISA® used to quantify Human TNFα with a sensitivity of 4.32 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x 96-well plates for bulk orders and 384-well for higher throughput
- Broad sample compatibility – works with CSF, serum, plasma, cell culture supernatants, and other biological fluids
- Cited in over 250 publications

查看别名

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

10 Images
Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)
  • sELISA

Supplier Data

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

Example of human TNF alpha standard curve in Sample Diluent NS.

The TNF alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)
  • sELISA

Lab

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

Example of human TNF alpha standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)
  • sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

Raw data value for example of TNF standard curve in Sample Diluent NS.

Raw data values are shown in the table

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)
  • sELISA

Supplier Data

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

Interpolated concentrations of native TNF alpha PBMC cell culture supernatant samples.

PBMC cells were stimulated with 1.5% PHA-M or vehicle control in 10F RPMI and incubated for 46 hours. The concentrations of TNF alpha were measured in duplicate and interpolated from the TNF alpha standard curves. Undiluted samples are PHA-M stimulated PBMC cell culture supernatant 10% and unstimulated PBMC cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was back calculated determined to be 7265.71 pg/mL in PHA-M stimulated PBMC cell culture supernatant and undetectable in the unstimulated PBMC control.

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)
  • sELISA

Lab

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

Human TNF alpha concentration was interpolated from the standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human TNF alpha ELISA kit (ab181421). Wild-type THP-1 and TNF alpha knockout THP-1 (ab273761) cells were assessed in duplicate (n=2). Cells were either treated with 100 ng/ml LPS for 16 h to induce expression of TNF alpha or not treated with LPS. Data are represented as the mean and error bars represent standard deviation."

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)
  • sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

Linearity of dilution - native TNF alpha.

Native TNF alpha was measured in biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)
  • sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

Linearity of dilution - recombinant TNF alpha.

Recombinant TNF alpha was spiked biological samples and diluted in a 2-fold dilution series in Sample Diluent NS. Twenty individual healthy human female/male donors were measured in duplicate for the presence of TNF alpha. All values were below the detectable range of the assay
NL – Non-Linear

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)
  • sELISA

Supplier Data

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

Human TNF alpha standard curve comparison.

Standard Curve comparison between human TNF alpha SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)
  • sELISA

Supplier Data

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

Interpolated concentrations of native TNF alpha in human PBMC cell culture supernatant samples.

PBMC cells were treated with 1.5% PHA-M for 46 hours in 10F RPMI. The concentrations of TNF alpha were measured in duplicates, interpolated from the TNF alpha standard curve and corrected for sample dilution. Undiluted samples are PHA-M treated PBMC cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was determined to be 7326.31 pg/mL in PHA-M treated PBMC cell culture supernatant.

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)
  • sELISA

Lab

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

关键信息

检测方法

Colorimetric

样品类型

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 4.32 pg/mL

范围

15.63 - 1000 pg/mL

检测时间

1h 30m

检测平台

Microplate

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Human TNF alpha ELISA Kit ab181421 is a rapid single-wash 90-min ELISA kit to measure Human TNF alpha in cerebral spinal fluid, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 4.32 pg/mL.

How the assay works

Human TNF alpha SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant human TNF alpha protein in serum, plasma, and cell culture supernatant samples only.

Human TNF alpha ELISA Kit ab181421 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human TNF alpha ELISA Kit ab181421

Human TNF alpha ELISA Kit ab181421 has been used to assess inflammation in different physio-pathological contexts such as obesity(1), coronary artery disease(2), and rheumatoid arthritis(3).

References:

(1)Shaohong Yu et al. 2023, PMID: 38156278,
(2)Yongshun Wang et al. 2023, PMID: 38054817,
(3)Yaju Tan et al. 2024, PMID: 37997635

Save your precious samples

Our 384-well Human TNF alpha ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human/Monkey TNF alpha Antibody Pair - BSA and Azide free ab241791
- Anti-TNF alpha antibody [EPR18565-29] - BSA and Azide free (Capture) ab242476
- Anti-TNF alpha antibody [EPR18565-33] - BSA and Azide free (Detector) ab242978

Related and recommended products

Human TNF alpha ELISA kit ab181421 is commonly used to measure TNF alpha as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Human IL-6 ELISA kit ab178013
- Human IL-8 ELISA kit ab214030
- Human IL-1 beta ELISA kit ab214025

TNF-alpha, also known as cachectin or TNFSF1A, is the prototypic ligand of the TNF superfamily which plays a central role in inflammation, apoptosis, proliferation, invasion, angiogenesis, metastasis and morphogenesis. It is expressed on macrophages, endothelial, epithelial and tumor cells as a 26kDa transmembrane protein. TNF-alpha is cleaved by proteolytic processing into six chains: (1) TNF membrane form, (2) Intracellular domain 1, (3) Intracellular domain 2, (4) C-domain 1, (5) C-domain 2 and (6) TNF soluble form. Signaling from TNF-alpha differs depending on the type of ligand initiating the signaling event (intracellular, membrane or soluble). As an example, the membrane form of TNF-alpha appears to mediate anti-tumorigenic therapeutic responses whereas the soluble ligand is linked to inflammation and proliferation.

style
left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "PBMC media", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "PBMC media", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
style
left-column

回收率

[ { "sample": "Cerebral Spinal Fluid", "range": "80 - 88 %", "average": "= 83" }, { "sample": "Cell culture supernatant", "range": "105 - 113 %", "average": "= 110" }, { "sample": "Serum", "range": "83 - 93 %", "average": "= 88" }, { "sample": "EDTA Plasma", "range": "95 - 104 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "89 - 110 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "86 - 97 %", "average": "= 91" } ]
style
left-column

规格

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab181421-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB181421-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB181421-CMP10" }, { "size":"2 x 1 Vial", "name":"Human TNFa Lyophilized Recombinant Protein", "number":"AB181421-CMP08" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB181421-CMP02" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB181421-CMP01" }, { "size":"1 x 600 µL", "name":"Human TNFa Capture Antibody", "number":"AB181421-CMP06" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB181421-CMP09" }, { "size":"1 x 600 µL", "name":"Human TNFa Detector Antibody", "number":"AB181421-CMP07" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB181421-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB181421-CMP11" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab181421-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB181421-CMP04" }, { "size":"2 x 10 Vial", "name":"Human TNFa Lyophilized Recombinant Protein", "number":"AB181421-CMP08" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB181421-CMP10" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB181421-CMP02" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB181421-CMP01" }, { "size":"10 x 600 µL", "name":"Human TNFa Capture Antibody", "number":"AB181421-CMP06" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB181421-CMP09" }, { "size":"10 x 600 µL", "name":"Human TNFa Detector Antibody", "number":"AB181421-CMP07" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB181421-CMP03" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB181421-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab181421-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB181421-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB181421-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB181421-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TNFa Lyophilized Recombinant Protein", "number":"AB181421-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"Human TNFa Capture Antibody", "number":"AB181421-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB181421-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB181421-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB181421-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"Human TNFa Detector Antibody", "number":"AB181421-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB181421-CMP09", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Tumor necrosis factor alpha (TNF alpha) also known as TNF-a is a cytokine with a molecular weight of approximately 17 kDa. This protein is expressed mainly by macrophages though other cells like T-cells and natural killer cells also produce it. TNF alpha helps in regulating immune responses and inflammatory reactions. Its expression is important in triggering cell apoptosis and modulating immune cell activities. TNF alpha exists in membrane-bound and soluble forms both having biologic activity.
Biological function summary

TNF alpha acts as an important regulator of inflammatory and immune responses. It is not part of a complex but interacts with TNF receptors to activate various signaling cascades. TNF alpha promotes leukocyte recruitment to sites of infection or injury by enhancing adhesion molecule expression on endothelial cells. Additionally it induces the production of other pro-inflammatory cytokines like IL-6 and IL-1 amplifying the immune response. Researchers often measure TNF alpha levels in samples using methods like ELISA or enzyme-linked immunosorbent assay and develop specific antibodies like anti-TNF alpha for experimental and therapeutic purposes.

Pathways

TNF alpha participates in the NF-kB and MAPK pathways significant for mediating inflammatory and immune responses. In the NF-kB pathway TNF alpha activates transcription factors that control the expression of genes involved in inflammation and cell survival. TNF alpha also interacts with proteins such as the TNF receptor 1 (TNFR1) and receptor-associated factors (TRAFs) further relaying signals within these pathways. There is extensive research employing animal models using mouse TNF-alpha and anti-TNF reagents to dissect these pathways’ intricacies.

TNF alpha substantially affects conditions like rheumatoid arthritis and Crohn's disease both characterized by chronic inflammation. Elevated levels of TNF alpha drive the inflammatory processes central to these diseases leading to tissue damage. Other proteins like IL-6 also play critical roles in these disorders and together with TNF alpha create a pro-inflammatory environment. Therapeutic agents such as anti-TNF treatments aim to mitigate these effects by specifically targeting TNF alpha activity.
style
left-column

产品实验方案

style
left-column

靶点信息

The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.
See full target information TNF
style
left-column

文献 (319)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 17: PubMed40573083

2025

Curcumin for Inflammation Control in Individuals with Type 2 Diabetes Mellitus and Metabolic Dysfunction-Associated Steatotic Liver Disease: A Randomized Controlled Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Metha Yaikwawong,Laddawan Jansarikit,Siwanon Jirawatnotai,Somlak Chuengsamarn

Naunyn-Schmiedeberg's archives of pharmacology : PubMed40514518

2025

Alpha-asarone relieves nasal inflammation, epithelial barrier damage, and mitochondrial damage in allergic rhinitis by inhibiting mitochondrial ROS via the SIRT1/PGC-1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Xu,Weimin Gao,Haitong Li,Xiaojuan Cao,Xiaohong Chen,Nannan Dong,Lingfang Wu,Yunzhen Luo

CytoJournal 22:38 PubMed40469707

2025

Methyltransferase 3-mediated m6A modification of Switch/sucrose non-fermenting-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 promotes mycobacterium tuberculosis-infected macrophage M1 polarization and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Chen,Hai Huang

Molecular diversity : PubMed40405028

2025

Novel pyrazole carboxylate derivatives as lonazolac bioisosteres with selective COX-2 inhibition: design, synthesis and anti-inflammatory activity.

Applications

Unspecified application

Species

Unspecified reactive species

Wael A A Fadaly,Ahmed Elshewy,Ahmed H A Abusabaa,Dina M E Amin,Hoda Khalifa Abdelhady,Haredy Hassan Haredy,Asmaa M Mahmoud,Nashwa A Ibrahim,Mohamed T M Nemr

American journal of translational research 17:3198-3206 PubMed40384998

2025

The effect of an intravenous analgesic pump with esketamine on postoperative pain and postpartum depression in women with cesarean section.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Xiao,Baoyi Su,Zhuoyu You,Hui Lu,Tuanming Huang

Inflammation : PubMed40372612

2025

LncRNA SNHG7/miR-181b-5p/TLR4 Activates Inflammation And Promotes Pyroptosis Through NF-κB Signaling in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zhang,Sheng-Jiang Xue,Feng Yang,Meng Xiao,Yong-Bo Tang,Ying Wu

American journal of translational research 17:2197-2209 PubMed40226016

2025

Development of a predictive model for the relationship between serum pan-immunoinflammatory index levels and scar formation in facial burn patients.

Applications

Unspecified application

Species

Unspecified reactive species

Di Xi,Hailun Yu,Tong Yu

Discover oncology 16:489 PubMed40198509

2025

Acinar cells modulate the tumor microenvironment through the promotion of M1 macrophage polarization via macrophage endocytosis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Congjia Ma,Wenbo Zhu,Xiulin Hu,Deli Wu,Xintong Zhao,Yiqi Du,Xiangyu Kong

Cell communication and signaling : CCS 23:165 PubMed40176138

2025

c-Ski is a novel repressor of NF-κB through interaction with p65 and HDAC1 in U937 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Peng,Ren-Ping Xiong,Bo Wang,Xing Chen,Ya-Lie Ning,Yan Zhao,Nan Yang,Jing Zhang,Chang-Hong Li,Yuan-Guo Zhou,Ping Li

Cell communication and signaling : CCS 23:141 PubMed40102920

2025

Increased melanin induces aberrant keratinocyte - melanocyte - basal - fibroblast cell communication and fibrogenesis by inducing iron overload and ferroptosis resistance in keloids.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangguang Shi,Xueyi Xia,Yang Xiao,Ying Zhang,Yiyi Gong,Yahui Chen,Chenyi Shi,Wei Wang,Jianlan Liu,Jia Huang,Mengguo Liu,Zhuoya Xu,Yanyun Ma,Mengkun Shi,Jiucun Wang,Wenyu Wu
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Assay Kits

AB214025

Human IL-1 beta ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB214030

Human IL-8 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

  • 5
  • 2

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com