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AB119608

人TIMP3 ELISA试剂盒(MIG-5)

Human TIMP3 ELISA Kit (MIG-5)

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(9 Publications)

Human TIMP3 ELISA Kit (MIG-5) is a Sandwich (quantitative) ELISA for the measurement of Human TIMP3 (MIG-5) in Human in Cell Culture Media, Biofluids samples.

查看别名

Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3

3 Images
Sandwich ELISA - Human TIMP3 ELISA Kit (MIG-5) (AB119608)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP3 ELISA Kit (MIG-5) (AB119608)

Representative standard curve using ab119608.

ELISA - Human TIMP3 ELISA Kit (MIG-5) (AB119608)
  • ELISA

Lab

ELISA - Human TIMP3 ELISA Kit (MIG-5) (AB119608)

TIMP3 measured in culture supernatants (duplicates +/- SD).

ELISA - Human TIMP3 ELISA Kit (MIG-5) (AB119608)
  • ELISA

Lab

ELISA - Human TIMP3 ELISA Kit (MIG-5) (AB119608)

Standard curve with background signal subtracted (duplicates; +/- SD).

关键信息

检测方法

Colorimetric

样品类型

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Saliva, Serum

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

< 2 pg/mL

范围

156 - 10000 pg/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Abcam's Human TIMP3 (MIG-5) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human TIMP3 (MIG-5) in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

A TIMP3 (MIG-5) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for TIMP3 (MIG-5) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human TIMP3 (MIG-5) amount of sample captured in plate.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4383 pg/mL", "standardDeviation": "271.74", "coefficientOfVariability": "= 6.2" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "248 pg/mL", "standardDeviation": "21.08", "coefficientOfVariability": "= 8.5" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "233 pg/mL", "standardDeviation": "18.21", "coefficientOfVariability": "= 7" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1474 pg/mL", "standardDeviation": "120.86", "coefficientOfVariability": "= 8.2" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1357 pg/mL", "standardDeviation": "92.27", "coefficientOfVariability": "= 6.8" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4049 pg/mL", "standardDeviation": "327.96", "coefficientOfVariability": "= 8.1" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119608-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Biotinylated anti-Human TIMP3 antibody", "number":"AB119608-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119608-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human TIMP3 antibody Microplate (12 x 8 wells)", "number":"AB119608-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119608-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119608-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119608-CMP07", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB119608-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119608-CMP08", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant Human TIMP3 standard", "number":"AB119608-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119608-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119608-CMP05", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Tissue inhibitor of metalloproteinases 3 (TIMP3) is widely recognized for its role in inhibiting the activity of matrix metalloproteinases (MMPs). It has an approximate mass of 24 kDa and is often referred to by its alternate name MIG. TIMP3 is expressed in various tissues especially those involved in extracellular matrix composition such as the kidney and lungs. Its expression helps maintain the balance between matrix deposition and degradation which is critical for normal tissue homeostasis.
Biological function summary

The function of TIMP3 extends beyond mere inhibition of MMPs; it is also involved in regulating angiogenesis and apoptosis. TIMP3 forms complexes with extracellular matrix components acting as an anchor that modulates cell-matrix interactions. This capacity to bind to matrix elements enhances its inhibitory activity against proteases making it an important regulator of extracellular matrix integrity.

Pathways

TIMP3 plays significant roles in extracellular matrix remodeling and the inflammatory response. It participates in pathways involving structural organization and signaling mediation such as the NF-kB signaling cascade. TIMP3 regulates proteins like TNF-alpha and MMPs which are central to these pathways balancing inflammatory responses and tissue remodeling processes.

The imbalance of TIMP3 activity has been linked to chronic obstructive pulmonary disease (COPD) and age-related macular degeneration (AMD). In COPD impaired regulation of ECM turnover by TIMP3 leads to destructive lung tissue remodeling. In AMD the regulation of angiogenesis and degradation of ECM around the retina by TIMP3 is disturbed influencing disease progression. Its interaction with proteins such as VEGF is critical in these pathological contexts where altered TIMP3 expression or function can exacerbate disease states.
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产品实验方案

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靶点信息

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
See full target information TIMP3
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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Ginekologia polska 94:400-406 PubMed35894478

2022

Association of tissue inhibitor of metalloproteinase-3 (TIMP-3) serum level and its genetic polymorphism with pregnancy outcome of patients undergoing in vitro fertilization and embryo transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Mahdis Meraji Masouleh Moghaddam,Farhad Mashayekhi,Ziba Zahiri,Akram Eidi

Veterinary ophthalmology 24:279-287 PubMed33834598

2021

Determining MMP-2 and MMP-9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Erotides Capistrano da Silva,Daniel J Gibson,Sunyoung Jeong,Kelli L Zimmerman,Kathryn M Smith-Fleming,Bianca da C Martins

The Journal of pathology 252:138-150 PubMed32666594

2020

In vitro stem cell modelling demonstrates a proof-of-concept for excess functional mutant TIMP3 as the cause of Sorsby fundus dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Heidi Hongisto,Jennifer M Dewing,David Rg Christensen,Jennifer Scott,Angela J Cree,Janika Nättinen,Juha Määttä,Antti Jylhä,Ulla Aapola,Hannu Uusitalo,Kai Kaarniranta,J Arjuna Ratnayaka,Heli Skottman,Andrew J Lotery

Experimental and therapeutic medicine 17:2433-2442 PubMed30906430

2019

Impact of several proinflammatory and cell degradation factors in patients with aortic valve stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Juris Lurins,Dace Lurina,Simons Svirskis,Zaiga Nora-Krukle,Peteris Tretjakovs,Vitolds Mackevics,Aivars Lejnieks,Venerando Rapisarda,Vincenzo Baylon

Acta ophthalmologica 97:84-90 PubMed30288950

2018

Imbalances in tissue inhibitors of metalloproteinases differentiate choroidal neovascularization from geographic atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Krogh Nielsen,Yousif Subhi,Christopher Rue Molbech,Line Lynge Nilsson,Mogens Holst Nissen,Torben Lykke Sørensen

Acta ophthalmologica 96:e27-e37 PubMed28391660

2017

Differential expression and localization of human tissue inhibitors of metalloproteinases in proliferative diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Abu El-Asrar,Ajmal Ahmad,Emilie Bittoun,Mohammad Mairaj Siddiquei,Ghulam Mohammad,Ahmed Mousa,Gert De Hertogh,Ghislain Opdenakker

Disease markers 2017:3510984 PubMed28348450

2017

A Novel Panel of Serum Biomarkers for MPM Diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

A Bonotti,R Foddis,S Landi,O Melaiu,C De Santi,L Giusti,E Donadio,F Ciregia,M R Mazzoni,A Lucacchini,M Bovenzi,M Comar,E Pantani,A Pistelli,A Cristaudo

Journal of the Royal Society, Interface 13: PubMed27466438

2016

In vitro response of macrophages to ceramic scaffolds used for bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela L Graney,Seyed-Iman Roohani-Esfahani,Hala Zreiqat,Kara L Spiller

International journal of biological sciences 9:557-63 PubMed23847438

2013

Circulating matrix metalloproteinase-2 and -9 enzyme activities in the children with ventricular septal defect.

Applications

Unspecified application

Species

Human

Kun-Shan Cheng,Yan-Chiou Liao,Mu-Yuan Chen,Tang-Ching Kuan,Yi-Han Hong,Li Ko,Wen-Yeh Hsieh,Chien-Liang Wu,Ming-Ren Chen,Chih-Sheng Lin
View all publications
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