人Thyroglobulin ELISA试剂盒(ab317775)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17.7 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Thyroglobulin ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Extract 8 2.2% 批次间 样品 n Mean SD CV% Extract 3 5.4% -
样品类型
Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
17.7 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
回收率
101 %
特定样本回收率 样品类型 平均% 范围 Serum 101 98% - 107% Tissue Extracts 104 98% - 113% Hep Plasma 95 94% - 98% EDTA Plasma 96 95% - 98% Cit plasma 100 98% - 101% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Thyroglobulin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thyroglobulin protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Tissue extracts. Quantitate Human Thyroglobulin with 17.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format. -
说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 10 x 96 tests 1 x 384 tests 1 x 96 tests 10X Human Thyroglobulin Capture Antibody 10 x 600µl 1 x 600µl 1 x 600µl 10X Human Thyroglobulin Detector Antibody 10 x 600µl 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 200ml 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit 0 x 0 unit 5X Cell Extraction Buffer PTR (ab193970) 2 x 50ml 1 x 50ml 1 x 10ml Antibody Diluent 4BI 10 x 6ml 1 x 6ml 1 x 6ml Human Thyroglobulin Lyophilized Recombinant Protein 20 vials 2 vials 2 vials Plate Seal 10 units 1 unit 1 unit Sample Diluent NS (ab193972) 0 x 0ml 0 x 0ml 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 10 units 0 x 0 unit 1 unit Stop Solution 1 x 120ml 2 x 12ml 1 x 12ml TMB Development Solution 1 x 120ml 2 x 12ml 1 x 12ml -
研究领域
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功能
Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). -
组织特异性
Thyroid gland specific. -
疾病相关
Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. -
序列相似性
Belongs to the type-B carboxylesterase/lipase family.
Contains 11 thyroglobulin type-1 domains. -
翻译后修饰
Sulfated tyrosines are desulfated during iodination. -
细胞定位
Secreted. - Information by UniProt
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别名
- AITD 3
- AITD3
- hTG
see all -
数据库链接
- Entrez Gene: 7038 Human
- Omim: 188450 Human
- SwissProt: P01266 Human
- Unigene: 654591 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317775于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of human Thyroglobulin standard curve in 1X Cell Extraction Buffer PTR.Example of human Thyroglobulin standard. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human Thyroglobulin in human thyroid extract.Interpolated concentration of native Thyroglobulin was measured in duplicate at different sample concentrations. Undiluted samples are 500 ng/mL human thyroid tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR. -
Example of human Thyroglobulin standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.Example of human Thyroglobulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of human Thyroglobulin in human thyroid extract in 96-well vs. 384-well plates.Interpolated concentration of native Thyroglobulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 400 ng/mL human thyroid tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR. -
Human Thyroglobulin ELISA Kit (ab317775)To learn more about the advantages of SimpleStep ELISA® kits see here.
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Human Thyroglobulin ELISA Kit (ab317775)To learn more about the advantages of recombinant antibodies see here.
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Human Thyroglobulin ELISA Kit (ab317775)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
数据表及文件
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SDS download
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Datasheet download
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