人Thrombomodulin ELISA试剂盒(CD141) (ab46508)
Key features and details
- Sensitivity: 0.31 ng/ml
- Range: 0.625 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Thrombomodulin ELISA试剂盒(CD141)
参阅全部 Thrombomodulin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 6 3.9% 批次间 样品 n Mean SD CV% Serum 6 9.8% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.31 ng/ml -
范围
0.625 ng/ml - 20 ng/ml -
回收率
109 %
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检测时间
1h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Thrombomodulin (CD141) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombomodulin (CD141) in serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for Thrombomodulin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Thrombomodulin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Thrombomodulin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Thrombomodulin present in the samples.
This kit will recognize both endogenous and recombinant Human Thrombomodulin.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-Thrombomodulin Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Control Silver 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl Thrombomodulin Microplate (12 x 8 well strips) 1 unit 2 units Thrombomodulin Standard (lyophilized) Yellow 2 vials 4 vials -
研究领域
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功能
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. -
组织特异性
Endothelial cells are unique in synthesizing thrombomodulin. -
疾病相关
Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
序列相似性
Contains 1 C-type lectin domain.
Contains 6 EGF-like domains. -
翻译后修饰
N-glycosylated.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
细胞定位
Membrane. - Information by UniProt
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别名
- AHUS 6
- AHUS6
- BDCA 3
see all -
数据库链接
- Entrez Gene: 7056 Human
- Omim: 188040 Human
- SwissProt: P07204 Human
- Unigene: 2030 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46508于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Thrombomodulin measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 2-16 fold.
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab46508 被引用在 18 文献中.
- Bauer C et al. Minimal Change Disease Is Associated With Endothelial Glycocalyx Degradation and Endothelial Activation. Kidney Int Rep 7:797-809 (2022). PubMed: 35497798
- Pomponio G et al. Tocilizumab in COVID-19 interstitial pneumonia. J Intern Med 289:738-746 (2021). PubMed: 33511686
- Ma L et al. Increased complement activation is a distinctive feature of severe SARS-CoV-2 infection. bioRxiv N/A:N/A (2021). PubMed: 33655244
- Marchetti M et al. Endothelium Activation Markers in Severe Hospitalized COVID-19 Patients: Role in Mortality Risk Prediction. TH Open 5:e253-e263 (2021). PubMed: 34235394
- Mehic D et al. Thrombomodulin in patients with mild to moderate bleeding tendency. Haemophilia 27:1028-1036 (2021). PubMed: 34628704