人Thrombin-Antithrombin Complex ELISA试剂盒(TAT) (ab108907)
Key features and details
- Sensitivity: 0.78 ng/ml
- Range: 1.48 ng/ml - 120 ng/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Thrombin-Antithrombin Complex ELISA试剂盒(TAT)
参阅全部 Thrombin-Antithrombin Complex 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.4% 批次间 样品 n Mean SD CV% Overall 10.4% -
样品类型
Cell culture supernatant, Milk, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.78 ng/ml -
范围
1.48 ng/ml - 120 ng/ml -
回收率
96 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Thrombin-Antithrombin Complex (TAT) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombin-Antithrombin Complex concentrations in plasma, milk and cell culture supernatants.
A Thrombin-Antithrombin Complex specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin-Antithrombin Complex specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin-Antithrombin Complex captured in plate.
Note: It is not recommended to perform spike recovery in plasma samples due to large amount of endogenous Anti-thrombin III (mg/ml range) which will saturate the capture antibody and interfere with the binding of the TAT standard
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Thrombin-Antithrombin Complex Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml Thrombin-Antithrombin Complex Microplate (12 x 8 well strips) 1 unit Thrombin-Antithrombin Complex Standard 1 vial -
研究领域
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相关性
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Antithrombin: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Antithrombin inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
细胞定位
Secreted -
别名
- antithrombin III
- AT3
- AT3D
see all -
数据库链接
- Entrez Gene: 462 Human
- Entrez Gene: 2147 Human
- Omim: 107300 Human
- Omim: 176930 Human
- SwissProt: P01008 Human
- SwissProt: P00734 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108907于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Sandwich ELISA - Thrombin-Antithrombin Complex (TAT) Human ELISA Kit
TAT measured in biological fluids, with background signal subtracted (duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve Using ab108907.
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab108907 被引用在 22 文献中.
- Mannes M et al. Combined Heterozygous Genetic Variations in Complement C2 and C8B: An Explanation for Multidimensional Immune Imbalance? J Innate Immun 15:412-427 (2023). PubMed: 36858027
- Anand RP et al. Design and testing of a humanized porcine donor for xenotransplantation. Nature 622:393-401 (2023). PubMed: 37821590
- de la Morena-Barrio ME et al. Contact pathway in surgical and transcatheter aortic valve replacement. Front Cardiovasc Med 9:887664 (2022). PubMed: 35935621
- Vincent LE et al. Association of Changes in Antithrombin Activity Over Time With Responsiveness to Enoxaparin Prophylaxis and Risk of Trauma-Related Venous Thromboembolism. JAMA Surg 157:713-721 (2022). PubMed: 35731524
- Li X et al. FGL2-MCOLN3-Autophagy Axis-Triggered Neutrophil Extracellular Traps Exacerbate Liver Injury in Fulminant Viral Hepatitis. Cell Mol Gastroenterol Hepatol 14:1077-1101 (2022). PubMed: 35926777