人TGFB3 ELISA试剂盒(ab272203)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.038 ng/ml
- Range: 0.38 ng/ml - 24 ng/ml
- Sample type: Cell culture media, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人TGFB3 ELISA试剂盒
参阅全部 TGF beta 3 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Extract 8 5.2% 批次间 样品 n Mean SD CV% Extract 3 11.1% -
样品类型
Tissue Extracts, Cell culture media -
检测类型
Sandwich (quantitative) -
灵敏度
0.038 ng/ml -
范围
0.38 ng/ml - 24 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Tissue Extracts 91 86% - 96% Cell culture media 100 89% - 107% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human TGFB3 ELISA kit (ab272200) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TGFB3 protein in cell culture supernatant and cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TGFB3 with 0.038 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Transforming growth factor beta-3 is a cytokine encoded by the TGFB3 gene. TGFB3 belongs to a larger family of proteins known as the transforming growth factor beta superfamily, which includes TGFB1 and 2, bone morphogenetic proteins, Growth Differentiation factors, inhibins and activins. TGFB3 is expressed as a proprotein containing a 277- amino acid latency-associated peptide (LAP) that remains non-covalently associated with TGFB3 after cleavage by the Golgi apparatus. Interaction with integrins removes the LAP, thus releasing a 112- amino acid active TGFB3. Active TGFB3 has many functions, including regulation of the embryogenesis and secondary palate development cell differentiation. The standard protein in this product is the active sequence of TGFB3.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human TGFB3 Capture Antibody 1 x 600µl 10X Human TGFB3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human TGFB3 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Involved in embryogenesis and cell differentiation. -
疾病相关
Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. -
序列相似性
Belongs to the TGF-beta family. -
细胞定位
Secreted. - Information by UniProt
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别名
- ARVD
- ARVD1
- FLJ16571
see all -
数据库链接
- Entrez Gene: 7043 Human
- Omim: 190230 Human
- SwissProt: P10600 Human
- Unigene: 592317 Human
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272203于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human TGFB3 standard curve in Sample Diluent NS.The TGFB3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked TGFB3 in human heart extract and RPMI cell culture media samples.The concentrations of TGFB3 were measured in duplicates, interpolated from the TGFB3 standard curves and corrected for sample dilution. Undiluted samples are as follows: heart extract 100 µg/mL, 50% RPMI containing 10% FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human TGFB3 ELISA Kit (ab272203)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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