人Testosterone free ELISA试剂盒(ab178663)
Key features and details
- Sensitivity: 0.06 pg/ml
- Range: 0.1 pg/ml - 42 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
人Testosterone free ELISA试剂盒
参阅全部 Testosterone 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sera 15 < 10% 批次间 样品 n Mean SD CV% Sera 10 < 10% -
样品类型
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Competitive -
灵敏度
>= 0.06 pg/ml -
范围
0.1 pg/ml - 42 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Free Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in serum and plasma.
A 96-well plate has been precoated with anti- Free Testosterone antibodies (solid-phase). Samples and the Free Testosterone-HRP conjugate are added to the wells, where Free Testosterone in the sample competes with the added Free Testosterone-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Free Testosterone in the sample and the intensity is measured at 450 nm.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Solution 1 x 50ml Anti-Testosterone IgG Coated Microplate (12 x 8 wells) 1 unit Cover Foil 1 unit Free Testosterone Control A 1 x 1ml Free Testosterone Control B 1 x 1ml Stop Solution 1 x 15ml Strip holder 1 unit Testosterone HRP Conjugate 1 x 15ml Testosterone Standard 0 - 0 pg/mL 1 x 1ml Testosterone Standard 1 - 0.2 pg/mL 1 x 1ml Testosterone Standard 2 - 1.0 pg/mL 1 x 1ml Testosterone Standard 3 - 4.0 pg/mL 1 x 1ml Testosterone Standard 4 - 20 pg/mL 1 x 1ml Testosterone Standard 5 - 100 pg/mL 1 x 1ml TMB Substrate Solution 1 x 15ml -
研究领域
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别名
- 17ß-hydroxyandrost-4-ene-3-one
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数据库链接
- SwissProt: P04278 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178663于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Competitive ELISA |
Use at an assay dependent concentration.
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说明 |
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Competitive ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab178663 被引用在 4 文献中.
- Itodo OA et al. Early Changes in Androgen Levels in Individuals with Spinal Cord Injury: A Longitudinal SwiSCI Study. J Clin Med 11:N/A (2022). PubMed: 36362788
- Yang Y et al. Cryptotanshinone alleviates polycystic ovary syndrome in rats by regulating the HMGB1/TLR4/NF-?B signaling pathway. Mol Med Rep 22:3851-3861 (2020). PubMed: 32901834
- Colombelli KT et al. Impairment of microvascular angiogenesis is associated with delay in prostatic development in rat offspring of maternal protein malnutrition. Gen Comp Endocrinol 246:258-269 (2017). PubMed: 28041790
- Constantino FB et al. The prostate response to prolactin modulation in adult castrated rats subjected to testosterone replacement. J Mol Histol 48:403-415 (2017). PubMed: 28988314