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AB178663

人Testosterone free ELISA试剂盒

Human Testosterone free ELISA Kit

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(4 Publications)

Human Testosterone free ELISA Kit is a competitive ELISA designed to quantify Human free testosterone with a sensitivity of 0.06 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.1 - 42  pg/mL

查看别名

17b hydroxy 4 androsten 3 one, 17鑴-hydroxyandrost-4-ene-3-one

1 Images
Competitive ELISA - Human Testosterone free ELISA Kit (AB178663)
  • cELISA

Supplier Data

Competitive ELISA - Human Testosterone free ELISA Kit (AB178663)

Representative standard curve using ab178663.

关键信息

检测方法

Colorimetric

样品类型

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

反应种属

Human

检测类型

Competitive

灵敏度

>= 0.06 pg/mL

范围

0.1 - 42 pg/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Abcam's Free Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in serum and plasma.

A 96-well plate has been precoated with anti- Free Testosterone antibodies (solid-phase). Samples and the Free Testosterone-HRP conjugate are added to the wells, where Free Testosterone in the sample competes with the added Free Testosterone-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Free Testosterone in the sample and the intensity is measured at 450 nm.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 10, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 15, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab178663-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"10X Wash Solution", "number":"AB178663-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover Foil", "number":"AB178663-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Testosterone IgG Coated Microplate (12 x 8 wells)", "number":"AB178663-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB178663-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB178663-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 5 - 100 pg/mL", "number":"AB178663-CMP15", "productcode":"" }, { "size":"1 x 1 mL", "name":"Free Testosterone Control A", "number":"AB178663-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB178663-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 4 - 20 pg/mL", "number":"AB178663-CMP14", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 1 - 0.2 pg/mL", "number":"AB178663-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 2 - 1.0 pg/mL", "number":"AB178663-CMP12", "productcode":"" }, { "size":"1 x 15 mL", "name":"Testosterone HRP Conjugate", "number":"AB178663-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"Free Testosterone Control B", "number":"AB178663-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 3 - 4.0 pg/mL", "number":"AB178663-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 0 - 0 pg/mL", "number":"AB178663-CMP10", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Testosterone also known as 17β-hydroxyandrost-4-en-3-one is a steroid hormone with a molecular mass of approximately 288.42 Da. It is primarily synthesized in the testes but also in smaller amounts in the ovaries and adrenal glands. Mechanically testosterone operates by binding to the androgen receptor a member of the nuclear receptor family which then modulates gene expression. Its synthesis is stimulated by luteinizing hormone (LH) which is released from the anterior pituitary gland.
Biological function summary

Testosterone plays an important role in the development of male secondary sexual characteristics such as increased muscle and bone mass and the growth of body hair. It's also part of the steroid hormone biosynthesis pathway influencing the production of estradiol through the aromatase enzyme. Testosterone contributes to the maintenance of reproductive tissues and is important for the modulation of mood and cognitive functions.

Pathways

Testosterone is integral to the HPG (hypothalamic-pituitary-gonadal) axis where it exerts feedback control by inhibiting the secretion of gonadotropin-releasing hormone (GnRH) and LH. Moreover testosterone is essential in the androgen receptor signaling pathway. This pathway involves other proteins like dihydrotestosterone (DHT) a potent androgen converted from testosterone via the 5α-reductase enzyme.

Low levels of testosterone commonly known as 'Low T' are linked to conditions like hypogonadism and certain metabolic syndromes. Testosterone levels can influence the risk of osteoporosis as sustained low levels reduce bone density. Additionally increased or decreased testosterone levels can pose risks to cardiovascular health with the target interacting with proteins such as sex hormone-binding globulin (SHBG) in these contexts.
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产品实验方案

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靶点信息

See full target information Testosterone
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical medicine 11: PubMed36362788

2022

Early Changes in Androgen Levels in Individuals with Spinal Cord Injury: A Longitudinal SwiSCI Study.

Applications

Unspecified application

Species

Unspecified reactive species

Oche Adam Itodo,Peter Francis Raguindin,Jens Wöllner,Inge Eriks-Hoogland,Xavier Jordan,Margret Hund-Georgiadis,Taulant Muka,Jürgen Pannek,Jivko Stoyanov,Marija Glisic

Molecular medicine reports 22:3851-3861 PubMed32901834

2020

Cryptotanshinone alleviates polycystic ovary syndrome in rats by regulating the HMGB1/TLR4/NF‑κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yijiao Yang,Ling Yang,Cao Qi,Guohua Hu,Longhui Wang,Zhuojun Sun,Xiaorong Ni

Journal of molecular histology 48:403-415 PubMed28988314

2017

The prostate response to prolactin modulation in adult castrated rats subjected to testosterone replacement.

Applications

Unspecified application

Species

Unspecified reactive species

Flávia B Constantino,Ana C L Camargo,Sérgio A A Santos,Ketlin T Colombelli,Laura F Martin,Marcia G Silva,Sérgio L Felisbino,Luis A Justulin

General and comparative endocrinology 246:258-269 PubMed28041790

2017

Impairment of microvascular angiogenesis is associated with delay in prostatic development in rat offspring of maternal protein malnutrition.

Applications

Unspecified application

Species

Unspecified reactive species

Ketlin T Colombelli,Sérgio A A Santos,Ana C L Camargo,Flávia B Constantino,Caroline N Barquilha,Jaqueline C Rinaldi,Sérgio L Felisbino,Luis A Justulin
View all publications
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