JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB285288

人TERT ELISA试剂盒

Human TERT ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human TERT ELISA Kit is a Sandwich ELISA for the measurement of Human TERT in in Biofluids, Cell/Tissue Extracts, Cell Culture Media samples.

查看别名

EST2, TCS1, TRT, TERT, Telomerase reverse transcriptase, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2

1 Images
ELISA - Human TERT ELISA Kit (AB285288)
  • ELISA

Supplier Data

ELISA - Human TERT ELISA Kit (AB285288)

Human TERT ELISA Kit.

Standard curve using Human TERT ELISA Kit ab285288 where R2 = 0.9888. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids

检测类型

Sandwich

Quantitative
灵敏度

< 0.094 ng/mL

范围

0.156 - 10 ng/mL

检测平台

Microplate (12 x 8 well strips)

style
left-column

搭配使用产品

Assay Kits

AB183361

Human Survivin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Survivin ELISA Kit (AB183361)

  • 0
  • 0
Assay Kits

AB283881

Human ErbB2 / HER2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)

  • 0
  • 0
Assay Kits

AB212159

Human Parkin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Parkin ELISA Kit (AB212159)

  • 0
  • 0
Primary Antibodies

AB32020

Anti-Telomerase reverse transcriptase antibody [Y182] (Overexpressed)

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Telomerase reverse transcriptase antibody [Y182] (Overexpressed) (AB32020)

  • 4
  • 9
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

This Telomerase reverse transcriptase (TERT) ELISA kit (ab285288, K4187) is a sandwich ELISA assay for the quantitative measurement of human TERT in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of TERT captured from the samples.

This product is manufactured by BioVision, an Abcam company and was previously called K4187 TERT (Human) ELISA Kit. K4187-100 is the same size as the 96 test size of ab285288.

style
left-column

规格

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285288-96Test", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285288-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285288-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample and Standard Dilution Buffer", "number":"AB285288-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285288-CMP04", "productcode":"" }, { "size":"5 x 1 Unit", "name":"Plate Sealer", "number":"AB285288-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285288-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285288-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285288-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard", "number":"AB285288-CMP07", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin-Detection antibody (Concentrated)", "number":"AB285288-CMP03", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285288-CMP09", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Telomerase reverse transcriptase also known as TERT is an enzyme with a molecular mass of about 127 kDa. It functions as a catalytic subunit of the telomerase complex which extends telomeres by adding the nucleotide sequence "TTAGGG" to the ends of chromosomes. TERT operates with a telomerase RNA component (TERC) to synthesize DNA using an RNA template a process called reverse transcription. TERT is expressed in embryonic tissues adult stem cells and cancer cells where rapid cell division occurs.
Biological function summary

TERT plays an important role in maintaining telomere length essential for protecting chromosome stability. The protein forms part of the telomerase holoenzyme complex working together with TERC to perform its function. Telomerase activity allows cells to bypass the Hayflick limit preventing senescence and enhancing replication potential. In addition to telomere maintenance TERT influences gene regulation and cellular proliferation.

Pathways

TERT holds significance in the telomere maintenance and signaling pathways. Telomere maintenance ensures genomic integrity and cellular longevity a process fundamental for tissue homeostasis and regeneration. TERT interacts with proteins such as dyskerin and TERC within these pathways. Its regulatory roles in these pathways also reveal interactions with other key proteins like hypothesized targets in the DNA damage response.

The upregulation of TERT is associated with cancer and degenerative diseases. Increased telomerase activity is a common feature in many cancers leading to uncontrolled cell proliferation. Additionally mutations in TERT and its associated components can contribute to disorders like dyskeratosis congenita a rare genetic condition resulting in bone marrow failure. In these conditions TERT interaction with proteins involved in chromosomal maintenance and repair becomes important for understanding disease mechanisms.
style
left-column

产品实验方案

style
left-column

靶点信息

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
See full target information TERT
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com