人Tenascin-C ELISA试剂盒(TNC)

产品详情

Human Tenascin-C ELISA Kit (TNC) ab213831 is a sandwich ELISA to measure Human Tenascin-C (TNC) in serum, EDTA plasma, heparin plasma, cell culture supernatant, urine with a sensitivity of 10 pg/ml.

How the assay works

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tenascin-C/TNC has been pre-coated onto 96-well plates. Standards (Expression system for standard (NSO; Immunogen sequence: G23-P625) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tenascin-C/TNC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Tenascin-C/TNC amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Tenascin-C ELISA Kit (TNC) ab213831 protocol summary

1. Add standard or sample to each well used. Incubate 37C
2. Add prepared biotin antibody to each well. Incubate at 37C
3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C
4. Add TMB to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450 nm

Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.