AB277081

人Tenascin C ELISA试剂盒

Human Tenascin C ELISA Kit

Recombinant
SimpleStep
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Human Tenascin C ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Tenascin C with a sensitivity of 27.672 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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HXB, TNC, Tenascin, TN, Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, JI, Myotendinous antigen, Neuronectin, Tenascin-C, TN-C

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Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

sELISA

Supplier Data

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

Interpolated concentrations of native Tenascin C in human serum and plasma samples.

The concentrations of Tenascin C were measured in duplicates, interpolated from the Tenascin C standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tenascin C concentration was determined to be 153.25 ng/mL in serum, 113.37 ng/mL in plasma (citrate), 108.74 ng/mL in plasma (EDTA), and 101.20 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

5% serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tenascin C concentration was determined to be 351.07 ng/mL with a range of 247.61 – 487.97 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

Example of human Tenascin C standard curve in Sample Diluent NS. The Tenascin C standard curve was prepared as described in Section 10.

The Tenascin C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 27.672 pg/mL

范围

312.5 - 20000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human Tenascin C ELISA kit (ab277081) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Tenascin C protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tenascin C with 27.67 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Tenascin C (TNC), is an extracellular matrix glycoprotein involved in tissue morphogenesis like embryogenesis, wound healing and tumorigenesis. Tenascin C is implicated in guidance of migrating neurons and axons during development, synaptic plasticity and neuronal regeneration. Tenascin C also as a role in neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. In tumors, Tenascin C stimulates angiogenesis by elongation, migration and sprouting of endothelial cell. Tenascin C shares 69.0%, 70.6%, 72.0%, and 97.3% sequence homology with mouse, rat, bovine, and monkey, respectively.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]

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回收率

[ { "sample": "Serum", "range": "88 - 106 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "96 - 99 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "84 - 94 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "83 - 106 %", "average": "= 93" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277081-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277081-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277081-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277081-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277081-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277081-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Tenascin C Lyophilized Recombinant Protein", "number":"AB277081-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Tenascin C Capture Antibody", "number":"AB277081-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB277081-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277081-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Tenascin C Detector Antibody", "number":"AB277081-CMP03", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Tenascin C also known as hexabrachion is an extracellular matrix glycoprotein with a mass of approximately 220-300 kDa depending on post-translational modifications. It shows high expression in areas undergoing tissue remodeling such as embryonic tissues wounds and tumors. It consists of repeating structural elements including epidermal growth factor-like repeats and fibronectin type III domains which facilitate its function in the context of tissue dynamics.

Biological function summary

The large hexamer known as Tenascin C plays a significant role in modulating cell adhesion migration and proliferation especially during developmental processes and injury responses. It incorporates into the existing extracellular matrix as a loose network which provides elasticity and supports cellular activities. This feature allows Tenascin C to interact with other matrix molecules and cell surface receptors to regulate tissue morphogenesis.

Pathways

Tenascin C actively participates in the Wnt and TGF-beta signaling pathways important for controlling cellular behavior in development and regeneration. It associates with integrins and glycoproteins to influence cell responses to signaling events in these pathways. By modulating these interactions Tenascin C can impact processes such as epithelial-to-mesenchymal transition and angiogenesis affecting tissue architecture and function.

Tenascin C exhibits a strong connection to cancer progression and chronic inflammatory conditions such as rheumatoid arthritis. Its levels rise in tumors and inflamed tissues where it interacts with other matrix components and cytokines like interleukin-6 (IL-6). These interactions contribute to a microenvironment that promotes tumor growth and persistence of inflammation highlighting its role in the pathophysiology of these diseases.

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产品实验方案

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靶点信息

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed : 19884327).

See full target information TNC

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:12028 PubMed38797735

2024

The serum tenascin C level is a marker of metabolic disorder-related inflammation affecting pancreatic cancer prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Katsuhiko Sato,Hayato Hikita,Minoru Shigekawa,Kazumasa Soma,Ryohei Yamauchi,Jihyun Sung,Seiya Kato,Yoichi Sasaki,Shinnosuke Kudo,Kenji Fukumoto,Kumiko Shirai,Kazuhiro Murai,Yuki Tahata,Teppei Yoshioka,Akira Nishio,Yoshinobu Saito,Takahiro Kodama,Yutaka Sasaki,Tomohide Tatsumi,Tetsuo Takehara

View all publications

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websiteProtocolBooklet

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人Tenascin C ELISA试剂盒

Human Tenascin C ELISA Kit

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台