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AB325447

Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular

Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular

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Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human Tau (phospho T217) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :68.6 - 50000 pg/mL with a sensitivity of 17.08 pg/mL
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Patent Pending

查看别名

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

6 Images
Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)
  • sELISA

Lab

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)
  • sELISA

Lab

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)

Standard curve comparison between the Chemiluminescent Human Tau (phospho T217) SimpleStep ELISA and colorimetric Human Tau (phospho T217) SimpleStep ELISA (ab318936) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)
  • sELISA

Lab

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)

Recombinant Tau (phospho T217) was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)
  • sELISA

Lab

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)

100 ng/mL of recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT181, Tau pS199, Tau pT231 and Tau pS396) were tested for specificity, and for interference with 10 ng/mL of recombinant Tau-A ligated with a peptide containing phosphorylated T217 (Tau pT217). The interpolated dilution-corrected values, expressed in % of input (for specificity) and in % of Tau pT217 control (for interference), are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)
  • sELISA

Lab

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)

Recombinant Tau (phospho T217) was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows : SH-SY5Y cell culture supernatant 12.5%, cell culture media (RPMI1640 containing 10% fetal bovine serum)100%, and CSF 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)
  • sELISA

Lab

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit, Chemiluminescent - Extracellular (AB325447)

Dynamic range comparison between the Chemiluminescent Human Tau (phospho T217) SimpleStep ELISA and colorimetric Human Tau (phospho T217) SimpleStep ELISA (ab318936).

关键信息

检测方法

Luminescent

样品类型

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 17.08 pg/mL

范围

68.6 - 50000 pg/mL

检测时间

55m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Tau (phospho T217) ELISA Kit, Chemiluminescent- Extracellular (ab325447) is a rapid single-wash sandwich 55-min ELISA to measure Human Tau (phospho T217) in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Cerebrospinal Fluid with a sensitivity of 17.08 pg/ml.

How the assay works

Human Tau (phospho T217) ELISA Kit, Chemiluminescent- Extracellular (ab325447) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human Tau (phospho T217) (ab325447) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.9" } ]
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回收率

[ { "sample": "Serum", "range": "80 - 87 %", "average": "= 84" }, { "sample": "Citrate plasma", "range": "76 - 89 %", "average": "= 82" }, { "sample": "EDTA Plasma", "range": "77 - 85 %", "average": "= 82" }, { "sample": "Heparin Plasma", "range": "77 - 87 %", "average": "= 82" }, { "sample": "Cell culture supernatant", "range": "81 - 92 %", "average": "= 86" }, { "sample": "Cerebral Spinal Fluid", "range": "104 - 113 %", "average": "= 108" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325447-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human Tau (phospho T217) Detector Antibody", "number":"AB325447-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325447-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Tau (phospho T217) Capture Antibody", "number":"AB325447-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325447-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325447-CMP08", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325447-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Tau (phospho T217) Lyophilized Recombinant Protein", "number":"AB325447-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB325447-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325447-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325447-CMP05", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MAPT phospho T217
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websiteProtocolBooklet
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