AB324328

Human Tau (phospho S396) ELISA Kit- Extracellular

SimpleStep
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Human Tau (phospho S396) ELISA Kit- Extracellular is a Sandwich ELISA for the measurement of Human Tau (phospho S396) - Extracellular in Human in Biofluids, Cell Culture Media samples.

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

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Sandwich ELISA - Human Tau (phospho S396) ELISA Kit- Extracellular (AB324328)

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit- Extracellular (AB324328)

Interpolated concentrations of native (A) human Tau (phospho S396) of 6 individual post-mortem human CSF donors measured in singlet and (B) human Tau (total, measured using ab273617) of the same 6 individual post-mortem human CSF donors measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit- Extracellular (AB324328)

Recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT181, Tau pT217, Tau pT231 and Tau pS199) were tested for specificity (each at 25,600 pg/mL), and for interference (each at 12,800 pg/mL) combined with 1,600 pg/mL of recombinant Tau-A ligated with a peptide containing phosphorylated S396 (Tau pS396). The interpolated dilution-corrected values, expressed in % of input (for specificity) and in % of Tau pS396 control (for interference), are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit- Extracellular (AB324328)

Interpolated concentration of native Tau (phospho S396) was measured in duplicate at different sample concentrations. Undiluted samples are 50% pooled post-mortem CSF. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit- Extracellular (AB324328)

Example of human Tau (phospho S396) standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit- Extracellular (AB324328)

Example of human Tau (phospho S396) standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid

反应种属

Human

检测类型

Sandwich

灵敏度

= 20.957 pg/mL

范围

50 - 3200 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human Tau (phospho S396) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau (phospho S396) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Cerebral Spinal Fluid. Quantitate Human Tau (phospho S396) with 20.957 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.1" } ]

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回收率

[ { "sample": "Serum", "range": "78 - 84 %", "average": "= 81" }, { "sample": "Citrate plasma", "range": "90 - 97 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "83 - 88 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "82 - 89 %", "average": "= 87" }, { "sample": "Cell culture supernatant", "range": "98 - 103 %", "average": "= 100" }, { "sample": "Cerebral Spinal Fluid", "range": "87 - 95 %", "average": "= 90" } ]

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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产品实验方案

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靶点信息

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT phospho S396

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websiteProtocolBooklet

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Human Tau (phospho S396) ELISA Kit- Extracellular